June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Rapamycin enhances the TNF-α-induced secretion of IL-6 and IL-8 by suppressing PDCD4 degradation in orbital fibroblasts
Author Affiliations & Notes
  • Won-Mo Lee
    Seong-Mo Eye Clinic, Dae Jeon, Republic of Korea
  • Jae Yon Won
    Ophthalmology and Visual Science, the Catholic University of Korea, Seoul, Republic of Korea
  • Ji-Sun Paik
    Ophthalmology and Visual Science, the Catholic University of Korea, Seoul, Republic of Korea
  • Won-Kyung Cho
    Ophthalmology and Visual Science, the Catholic University of Korea, Seoul, Republic of Korea
  • Eun-Hye Oh
    Institute of Hansen's Disease, The Catholic University of Korea, Seoul, Republic of Korea
    Department of Pathology, The Catholic University of Korea, Seoul, Republic of Korea
  • Seong-Beom Lee
    Institute of Hansen's Disease, The Catholic University of Korea, Seoul, Republic of Korea
    Department of Pathology, The Catholic University of Korea, Seoul, Republic of Korea
  • Suk-Woo Yang
    Ophthalmology and Visual Science, the Catholic University of Korea, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships Won-Mo Lee, None; Jae Yon Won, None; Ji-Sun Paik, None; Won-Kyung Cho, None; Eun-Hye Oh, None; Seong-Beom Lee, None; Suk-Woo Yang, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5907. doi:
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      Won-Mo Lee, Jae Yon Won, Ji-Sun Paik, Won-Kyung Cho, Eun-Hye Oh, Seong-Beom Lee, Suk-Woo Yang; Rapamycin enhances the TNF-α-induced secretion of IL-6 and IL-8 by suppressing PDCD4 degradation in orbital fibroblasts. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5907.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To investigate the effects of rapamycin on the TNF-α-induced secretion of interleukin (IL)-6 and IL-8 in orbital fibroblasts and its possible mechanism.

Methods: Orbital fibroblasts were obtained from patients with thyroid-associated ophthalmopathy. IL-6 and IL-8 levels were measured by enzyme-linked immunosorbent assays. The down-regulation of PDCD4 (program cell death 4) was performed by PDCD4 siRNA transfection.

Results: Rapamycin significantly enhanced TNF-α-induced IL-6 and IL-8 secretion from orbital fibroblasts. Down-regulation of PDCD4 by PDCD4 siRNA transfection reduced TNF-α-induced IL-6 and IL-8 secretion from orbital fibroblasts. In addition, TNF-α was found to promote the mTOR-dependent proteasome-mediated degradation of PDCD4. Rapamycin increased PDCD4 expression via the inhibition of TNF-α-induced PDCD4 degradation in orbital fibroblasts.

Conclusions: Rapamycin enhances the TNF-α-induced secretion of IL-6 and IL-8 by suppressing PDCD4 degradation in orbital fibroblasts.

Keywords: 631 orbit  
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