June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Expression of GRα mRNA and NF-kB in orbital fat and extraocular muscle in Thyroid Eye Disease
Author Affiliations & Notes
  • Ivana Romero-Kusabara
    Ophthalmology, Santa Casa of Sao Paulo, Sao Paulo, Brazil
    Molecular Medicine Laboratoty, Physiology, Santa Casa of Sao Paulo, Sao Paulo, Brazil
  • Adriano Cury
    Endocrinology, Santa Casa of Sao Paulo, Sao Paulo, Brazil
    Molecular Medicine Laboratoty, Physiology, Santa Casa of Sao Paulo, Sao Paulo, Brazil
  • José Vital Filho
    Ophthalmology, Santa Casa of Sao Paulo, Sao Paulo, Brazil
  • Nilza Scalissi
    Endocrinology, Santa Casa of Sao Paulo, Sao Paulo, Brazil
  • Keli Melo
    Molecular Medicine Laboratoty, Physiology, Santa Casa of Sao Paulo, Sao Paulo, Brazil
  • Giovanni Demartino
    Molecular Medicine Laboratoty, Physiology, Santa Casa of Sao Paulo, Sao Paulo, Brazil
  • Carlos Longui
    Endocrinology, Santa Casa of Sao Paulo, Sao Paulo, Brazil
    Molecular Medicine Laboratoty, Physiology, Santa Casa of Sao Paulo, Sao Paulo, Brazil
  • Murilo Melo
    Molecular Medicine Laboratoty, Physiology, Santa Casa of Sao Paulo, Sao Paulo, Brazil
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5912. doi:
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      Ivana Romero-Kusabara, Adriano Cury, José Vital Filho, Nilza Scalissi, Keli Melo, Giovanni Demartino, Carlos Longui, Murilo Melo; Expression of GRα mRNA and NF-kB in orbital fat and extraocular muscle in Thyroid Eye Disease. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5912.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: In this study we compared GRα mRNA and NF-kB in Thyroid Eye Disease’s (TED) orbital fat (OF) and extraocular muscle (EOM) with normal OF and EOM.

Methods: A prospective study was performed at the Ophthalmology Unit of Santa Casa of Sao Paulo, Brazil. We evaluated 34 TED patients and 42 healthy controls. OF was harvested from 34 TED and 25 controls. EOM biopsy was obtained from 33 TED and 20 controls. Samples were examined by real time PCR. Methods of statistical analysis were choosen depending on the type of the data, with the level of statistical significance as p value <0.05.

Results: A higher level of GRα mRNA was observed in TED muscle with median (p25-p75)=213.39 (96.91-376.78) compared to controls muscle with median (p25-p75)=60.99 (32.28-131.00), (p<0.001). The same behavior was seen when NF-kB was analysed in TED muscle with median (p25-p75)=223.49 (30.57-520.23) compared to controls muscle with median (p25-p75)=8.39 (6.40-30.04), (p<0.001). However, in OF samples, GRα mRNA and NF-kB were similar among groups. GRα/NF-kB ratio in TED EOM was 0.73 (0.34-5.18) and in controls EOM was 5.19 (3.73-8.05), (p=0.005).

Conclusions: GRα mRNA and NF-kB levels differed among tissues involved in the pathophysiology of Graves Orbitopathy. In TED OF samples there was no difference in gene expression compared to controls. In the other hand, in TED muscles samples, the levels of GRα mRNA and NF-kB were higher than controls.

Keywords: 631 orbit • 533 gene/expression • 739 transcription factors  
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