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Zhenzhen Liu, Mingxing Wu, Yi Zhu, Wanjun Zhang, Xuhua Tan, Shan Huang, Fu Shang, Yizhi Liu, Lab of Cataract Prevention and Treatment, Zhongshan Ophthalmic Center; Effects of basic FGF on the regeneration of lens in New Zealand Albino rabbits. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5942.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate effects of basic FGF on the regeneration of lens in New Zealand Albino rabbits.
Endocapsular lens extraction was performed by phacoemulsification and irrigation/aspiration with intravitreal injection of basic FGF(2.5µg/eye, FGF-low or 5µg/eye, FGF-high) in young adult (2-3 months of age) New Zealand albino rabbits. The animals were evaluated postoperatively with slit lamp biomicroscopy and photography at regular intervals: days1, 2, 3 and weeks1, 3, 4, 5 and 7. Each time the lens regeneration that filled the capsular bag was photographed and measured with the pupil maximally dilated. The lenses were then dissected and weighed at week 7. The regenerated lenses and natural control lenses were fixed and embedded in paraffin, stained with hematoxylin and eosin.
Postoperatively, lens regeneration was first noted at 2 weeks both in the control group and the FGF groups. By the 7-week postoperative examination, the regenerated lens in the control appeared with severe opacity in the periphery, while the regenerated lenses were more transparent, especially in the FGF-low group. When compared to contralateral lenses, regenerative lenses weighed 65% in the control, and weighed 40%, 79% respectively in the FGF-low and the FGF-high group. HE staining showed that in the FGF-low group, the remnant lens epithelial cells differentiate at the lens capsule equator and new lens fibers form in a concentric pattern in a manner similar to that observed in natural lenses.
This experimental model is the first to show that intra-vitreous injection of basic FGF may help maintain transparency of regenerated lens, and may accelerate regeneration of lens materials.
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