June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
FGF2 Antagonizes the TGFβ2-Induced Aberrant Expression of Tropomyosin and α-Smooth Muscle Actin in Mouse and Human Lens Epithelial Cells and Has a Role in Posterior Capsule Opacity
Author Affiliations & Notes
  • Eri Kubo
    Department of Ophthalmology, Kanazawa Medical University, Ishikawa, Japan
  • Nailia Hasanova
    Department of Ophthalmology, Kanazawa Medical University, Ishikawa, Japan
  • Hiromi Osada
    Department of Ophthalmology, Kanazawa Medical University, Ishikawa, Japan
  • Etsuko Kiyokawa
    Department of Oncogenic Pathology, Kanazawa Medical University, Ishikawa, Japan
  • Hiroshi Sasaki
    Department of Ophthalmology, Kanazawa Medical University, Ishikawa, Japan
  • Nigar Fatma
    Department of Ophthalmology, University of Nebraska Medical Center, Omaha, NE
  • Dhirendra Singh
    Department of Ophthalmology, University of Nebraska Medical Center, Omaha, NE
  • Footnotes
    Commercial Relationships Eri Kubo, None; Nailia Hasanova, None; Hiromi Osada, None; Etsuko Kiyokawa, None; Hiroshi Sasaki, None; Nigar Fatma, None; Dhirendra Singh, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5943. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Eri Kubo, Nailia Hasanova, Hiromi Osada, Etsuko Kiyokawa, Hiroshi Sasaki, Nigar Fatma, Dhirendra Singh; FGF2 Antagonizes the TGFβ2-Induced Aberrant Expression of Tropomyosin and α-Smooth Muscle Actin in Mouse and Human Lens Epithelial Cells and Has a Role in Posterior Capsule Opacity. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5943.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: FGF2 and TGFβ2 families are known to play an important role in regulating posterior capsule opacity (PCO) after cataract surgery. Using an in vivo rodent PCO model, we earlier demonstrated that the expression of tropomyosin (Tpm)1α/2β was aberrantly upregulated in remodeling the actin cytoskeleton during (epithelial-mesenchymal transition) EMT of lens epithelialcells (LECs). In the present study, we explored the effect of FGF2 and TGFβ2 on Tpm1α/2β and α-smooth muscle actin (αSMA) using mouse LECs from Peroxiredoxin 6-deficient (Prdx6-/-) and wild-type (Prdx6+/+) mice coupled with a rat PCO model system.

Methods: Prdx6-/- LECs overexpressing Tpm1α/2β, and Prdx6+/+ LECs with low expression of Tpm1α/2β were used. LECs growing in DMEM containing 0.5% bovine serum albumin were treated with 0 to 10ng/ml of FGF-2 and TGFβ-2. MTS and TUNEL assays were used to detect cell viability and apoptotic cell death. Expression of several genes related to lens EMT were examined using real-time PCR and Western analysis using their corresponding specific probes. All animal experiments were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Extracapsular lens extractions (ECLEs) were performed in Sprague Dawley rats to generate the rat PCO model.

Results: After treatment with TGFβ2, expression of Tpm1α/2β and αSMA were upregulated in Prdx6+/+ LECs, and cell viability was decreased. In contrast, with treatment with FGF-2 in the presence or absence of TGFβ2, expression of Tpm1α/2β and αSMA were markedly decreased in Prdx6-/- LECs and cell viability was increased. FGF2 treatment of also blunted the formation of stress fiber in LECs that overexpressed Tpm1α/2β. FGFR2 was localized in regenerated lens fiber in rat PCO model as evidenced by immunohistochemical analyses.

Conclusions: FGF-2 inhibits the TGF-β2-mediated induction of Tpm1α/2β and αSMA. The findings may help to clarify the condition of reprogramming of the actin cytoskeleton during morphogenetic EMT cell proliferation and fiber regeneration in PCO. Consideration of the balance of FGF-2 and EMT regulation may provide a clue to a method of postponing PCO.

Keywords: 652 posterior capsular opacification (PCO) • 512 EMT (epithelial mesenchymal transition) • 445 cataract  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×