June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Expression of NOD-like receptors(NLRs) and NODs in corneal diseases
Author Affiliations & Notes
  • Na-Kyung Ryoo
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
    Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • Jung Hwa Ko
    Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • Hyun Ju Lee
    Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • Mee Kum Kim
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • Won Ryang Wee
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
  • Joo Youn Oh
    Ophthalmology, Seoul National University Hospital, Seoul, Republic of Korea
    Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships Na-Kyung Ryoo, None; Jung Hwa Ko, None; Hyun Ju Lee, None; Mee Kum Kim, None; Won Ryang Wee, None; Joo Youn Oh, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 5993. doi:
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      Na-Kyung Ryoo, Jung Hwa Ko, Hyun Ju Lee, Mee Kum Kim, Won Ryang Wee, Joo Youn Oh; Expression of NOD-like receptors(NLRs) and NODs in corneal diseases. Invest. Ophthalmol. Vis. Sci. 2013;54(15):5993.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To evaluate the expression and potential implication of NLRs and NODs in corneal diseases

 
Methods
 

We investigated the transcription levels of IL-1β, NLRs, and NODs in human cornea obtained from individuals undergoing penetrating keratoplasty (PKP) for different underlying diseases. Whole human cornea (corneal buttons) removed during PKP were collected. Specimen were classified into four different groups depending on patients’ history and pre-operative clinical impression : 1) no inflammation (keratoconus), 2) infection, 3) allograft rejection, and 4) autoimmune inflammation. RNA were extracted from the tissue. cDNA was generated by reverse transcription and subjected to quantitative real-time polymerase chain reaction (PCR). Expression of NLR family members—Nod1, Nod2, NLRP1, NLRP3—and proinflammatory cytokine IL-1β were assessed and compared between the groups.

 
Results
 

In the corneas without inflammation, IL-1β was not expressed. There were no increase in Nod1, Nod2, NLRP1 expression compared to normal controls. In contrast, in the three groups of infection, allograft rejection, and autoimmune inflammation, there were a marked increase in NLRP3 and Nod2, while a small increase in NLRP1 and no increase in Nod1 was noted.

 
Conclusions
 

These data suggest that NLRP3-Nod2 axis may be involved in the pathogenesis of corneal disease.

 
 
Representative figures of each group: 1) No inflammation, 2) Infection, 3) Allograft rejection, and 4) Autoimmune inflammation
 
Representative figures of each group: 1) No inflammation, 2) Infection, 3) Allograft rejection, and 4) Autoimmune inflammation
 
Keywords: 480 cornea: basic science  
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