June 2013
Volume 54, Issue 15
ARVO Annual Meeting Abstract  |   June 2013
Updated Fluorophotometry Technique Correlates With Signs and Symptoms of Dry Eye Better Than Schirmer’s Test
Author Affiliations & Notes
  • Colleen Heckley
    R&D, Ora, Inc., Andover, MA
  • Endri Angjeli
    R&D, Ora, Inc., Andover, MA
  • Keith Lane
    R&D, Ora, Inc., Andover, MA
  • Canice Ahearn
    R&D, Ora, Inc., Andover, MA
  • Donna Welch
    Ora, Inc., Andover, MA
  • George Ousler
    Ora, Inc., Andover, MA
  • Footnotes
    Commercial Relationships Colleen Heckley, Ora, Inc. (E); Endri Angjeli, Ora, Inc. (E); Keith Lane, Ora, Inc. (E); Canice Ahearn, Ora Inc (E); Donna Welch, Ora, inc. (E); George Ousler, Ora, Inc. (E)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 6043. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Colleen Heckley, Endri Angjeli, Keith Lane, Canice Ahearn, Donna Welch, George Ousler; Updated Fluorophotometry Technique Correlates With Signs and Symptoms of Dry Eye Better Than Schirmer’s Test. Invest. Ophthalmol. Vis. Sci. 2013;54(15):6043.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Dry eye disease (DED) is associated with epithelial desiccation and therefore methods of quantifying tear production are critical to the clinician. The Schirmer’s test has remained the gold standard for evaluating tear production despite the fact that it has been shown to be crude, non-reproducible, and invasive to the tear film. Fluorophotometry measures tear production in a less invasive manner by accurately capturing the rate of fluorescien dye dilution from the tear film. Still, methods for performing fluorophotometry assessments vary between investigators, and the value of the metric for use clinically has been disputed. In this study, we compared an improved fluorophotometry technique to the Schirmer’s test to evaluate correlations of these measures with traditional signs and symptoms of dry eye disease (staining, ocular discomfort).


The following endpoints were assessed and correlations calculated on data from 11 subjects with DED: demographics, symptoms, quality of life (QoL), tear film break up time, Schirmer’s test scores (ST), improved tear fluorophotometry (F), fluorescein staining, corneal sensitivity, lid redness, and meibomian gland measurements. This fluorophotometry method varies from previously reported procedures through alterations in fluorescien drop volume, fluorescien drop concentration, and scan initiation in relation to the subject’s blink.


Fluorophotometry scores correlated well with both signs and symptoms of dry eye in this study, while Schirmer’s test did not correlate with any signs or symptoms measured. Higher flow rates (tear turnover rates) were associated with lower ocular discomfort scores and lower fluorescein staining (R=-0.81, p=0.005 for ocular discomfort. R=-0.17, p=0.05 for central staining).


Results suggest that when using this modified methodology, fluorophotometry may be a better tool for measuring aqueous secretion than the Schirmer’s test, based on superior correlations with traditional signs and symptoms of dry eye disease. Improving the methodology for performing fluorophotometry assessments is important to ensuring the greatest possible accuracy of the measurement.

Keywords: 486 cornea: tears/tear film/dry eye  

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.