June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Expression Of VEGF-A Gene In Normal Retina And In PVR Retina
Author Affiliations & Notes
  • Claudio Azzolini
    Morphological and Surgical Sciences, University of Insubria - Circolo Hospital, Varese, Italy
  • Ilaria Pagani
    Department of Clinical and Experimental Medicine, University of Insubria, Varese, Italy
  • Davide Borroni
    Morphological and Surgical Sciences, University of Insubria - Circolo Hospital, Varese, Italy
    Department of Clinical and Experimental Medicine, University of Insubria, Varese, Italy
  • Cristina Pirrone
    Department of Clinical and Experimental Medicine, University of Insubria, Varese, Italy
  • Diana Pigni
    Department of Clinical and Experimental Medicine, University of Insubria, Varese, Italy
  • Muna Al Oum
    Morphological and Surgical Sciences, University of Insubria - Circolo Hospital, Varese, Italy
  • Riccardo Vinciguerra
    Morphological and Surgical Sciences, University of Insubria - Circolo Hospital, Varese, Italy
  • Simone Donati
    Morphological and Surgical Sciences, University of Insubria - Circolo Hospital, Varese, Italy
  • Giovanni Porta
    Department of Clinical and Experimental Medicine, University of Insubria, Varese, Italy
  • Footnotes
    Commercial Relationships Claudio Azzolini, None; Ilaria Pagani, None; Davide Borroni, None; Cristina Pirrone, None; Diana Pigni, None; Muna Al Oum, None; Riccardo Vinciguerra, None; Simone Donati, None; Giovanni Porta, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 6201. doi:https://doi.org/
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      Claudio Azzolini, Ilaria Pagani, Davide Borroni, Cristina Pirrone, Diana Pigni, Muna Al Oum, Riccardo Vinciguerra, Simone Donati, Giovanni Porta; Expression Of VEGF-A Gene In Normal Retina And In PVR Retina. Invest. Ophthalmol. Vis. Sci. 2013;54(15):6201. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Vascular Endothelial Growth Factor-A, VEGF-A, is a key angiogenesis protein in embryonic development and in adult tissues. It is also involved in the pathogenesis of different human diseases including eye disorders, like proliferative vitreoretinopathy (PVR). Both in normal tissues expression and in PVR VEGF-A is regulated by p53 family members. This protein induces cellular differentiation regulating division in staminal cells, leading to asymmetric division, while its loss of function leads to the symmetric division, resulting in cellular growth.

Methods: We evaluated expression levels of p53, p63, p73 and VEGF-A genes through quantitative Real-time reverse-transcriptase PCR analysis in twelve retinal PVR samples, taken during necessary retinectomies, and healthy human retina controls included in paraffin.

Results: We showed VEGF-A over-expression in samples affected by a marked proliferative vitreoretinopathy compared with healthy human retina. VEGF-A is less expressed in PVR tissues detached from a long time from retina. Controls showed a basal levels of VEGF-A expression. p53 and p73 seems to co-expresses with VEGF-A while p63 is down-regulated in tissues with high levels of VEGF-A.

Conclusions: The increase of VEGF-A levels in PVR tissues promotes differentiation and cell survival and decreases apoptosis suggesting its role in proliferation of retinal cells. These analysis could be critical for the studies aimed to novel therapies for retinal diseases.

Keywords: 655 proliferative vitreoretinopathy • 603 Muller cells • 533 gene/expression  
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