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Claudio Azzolini, Ilaria Pagani, Davide Borroni, Cristina Pirrone, Diana Pigni, Muna Al Oum, Riccardo Vinciguerra, Simone Donati, Giovanni Porta; Expression Of VEGF-A Gene In Normal Retina And In PVR Retina. Invest. Ophthalmol. Vis. Sci. 2013;54(15):6201.
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Vascular Endothelial Growth Factor-A, VEGF-A, is a key angiogenesis protein in embryonic development and in adult tissues. It is also involved in the pathogenesis of different human diseases including eye disorders, like proliferative vitreoretinopathy (PVR). Both in normal tissues expression and in PVR VEGF-A is regulated by p53 family members. This protein induces cellular differentiation regulating division in staminal cells, leading to asymmetric division, while its loss of function leads to the symmetric division, resulting in cellular growth.
We evaluated expression levels of p53, p63, p73 and VEGF-A genes through quantitative Real-time reverse-transcriptase PCR analysis in twelve retinal PVR samples, taken during necessary retinectomies, and healthy human retina controls included in paraffin.
We showed VEGF-A over-expression in samples affected by a marked proliferative vitreoretinopathy compared with healthy human retina. VEGF-A is less expressed in PVR tissues detached from a long time from retina. Controls showed a basal levels of VEGF-A expression. p53 and p73 seems to co-expresses with VEGF-A while p63 is down-regulated in tissues with high levels of VEGF-A.
The increase of VEGF-A levels in PVR tissues promotes differentiation and cell survival and decreases apoptosis suggesting its role in proliferation of retinal cells. These analysis could be critical for the studies aimed to novel therapies for retinal diseases.
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