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Kamesh Dhamodaran, Reshma Shetty, Murali Subramani, Vinay Patil, Bhujang Shetty, Debashish Das, Stem Cell Research Laboratory; Identification and expression of candidate genes in pseudoexfoliation. Invest. Ophthalmol. Vis. Sci. 2013;54(15):6241.
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To identify the biomarker for early diagnosis of developing pseudoexfoliation with or without glaucoma.
After informed consent, fifteen samples of aqueous humor and lens was collected from patients who underwent surgery for PEX, PEX with glaucoma or glaucoma alone. The samples from cataract patients (Control) without any history of glaucoma and/or PEX. Molecular analysis by mRNA expression was done by quantitative PCR for the following genes LOXL1, CLU, MYOC, OPTN and Elvovl5.
Interestingly, qPCR analysis shows two fold increase of Clusterin expression was identified in aqueous humor and not in lens from PEX patients compared to pseudoexfoliation with glaucoma (XFG) and isolated glaucoma (POAG). Also well known susceptible LOXL1 gene was expressed in higher levels on both PEX and XFG patients aqueous humor consistent with previous reports. On the other hand, POAG or Normal tension glaucoma (NTG) marker ELOVL5 was observed in aqueous humor of PEX and XFG patients. As we expect MYOC shows predominant expression in aqueous humor from glaucoma patients.
In this study we showed that pseudoexfoliation candidate gene expression (LOXL1, CLU) pattern in patients with aqueous humor and lens. We are also confirming that higher expression of Myoclin in POAG patients. Finally we conclude that LOXL1, CLU, MYOC, OPTN and Elvovl5 as a marker for identifying predisposition of PEX and developing glaucoma.
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