June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Periostin Promotes the Generation of Fibrous Membranes in Proliferative Vitreoretinopathy
Author Affiliations & Notes
  • Keijiro Ishikawa
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Shigeo Yoshida
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Shintaro Nakao
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Takahito Nakama
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Takeshi Kita
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Yukio Sassa
    Ophthalmology, Kyushu University, Fukuoka, Japan
    Ophthalmology, Fukuoka University Chikushi Hospital, Fukuoka, Japan
  • Hiroshi Enaida
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Yuji Oshima
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Toshihiro Kono
    Ophthalmology, Fukuoka University Chikushi Hospital, Fukuoka, Japan
  • Tatsuro Ishibashi
    Ophthalmology, Kyushu University, Fukuoka, Japan
  • Footnotes
    Commercial Relationships Keijiro Ishikawa, None; Shigeo Yoshida, None; Shintaro Nakao, None; Takahito Nakama, None; Takeshi Kita, None; Yukio Sassa, None; Hiroshi Enaida, None; Yuji Oshima, None; Toshihiro Kono, None; Tatsuro Ishibashi, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 6253. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Keijiro Ishikawa, Shigeo Yoshida, Shintaro Nakao, Takahito Nakama, Takeshi Kita, Yukio Sassa, Hiroshi Enaida, Yuji Oshima, Toshihiro Kono, Tatsuro Ishibashi; Periostin Promotes the Generation of Fibrous Membranes in Proliferative Vitreoretinopathy. Invest. Ophthalmol. Vis. Sci. 2013;54(15):6253.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract
 
Purpose
 

We previously reported the increased expression of periostin in patients with proliferative vitreoretinopathy (PVR) and that periostin enhanced the proliferation, adhesion, migration and collagen synthesis through integrin αV-mediated FAK and AKT phosphorylation of retinal pigment epithelial (RPE) cells. The purpose of this study was to investigate, in vivo, the suppressive effect of periostin on the pathogenesis of fibrous membrane with PVR.

 
Methods
 

We generated mouse PVR model using C57BL/6 WT and periostin knockout mice according to the previous report. Masson’s trichrome staining and immunofluorescence staining with collagen type I antibody were performed to evaluate the formation of the fibrous membranes. We observed the effect of periostin neutralizing Ab (NAb) on rabbit PVR model that involves injecting RPE cells in the vitreous. Electroretinography and histological examination were performed to evaluate the retinal function after intravitreal injections of periostin NAb.

 
Results
 

The findings of Masson’s trichrome staining showed scarce collagen deposition in the lesions of periostin knockout mice compared to WT mice. The areas of fibrous membrane were significantly decreased in the RPE/choroidal flat mount preparations in periostin knockout mice compared to WT mice (p <0.05). Administration of periostin NAb significantly suppressed the progression of PVR from day 5 to day 21. The mean amplitude of the photopic b wave in the rabbit eye treated with periostin NAb was not significantly different from that of the control eye. The histological structure of the rabbit retina in the eyes injected with periostin NAb appeared normal.

 
Conclusions
 

The present study identified periostin as a promising therapeutic target for PVR.

 
 
Intravitreal injections of periostin NAb and control IgG were performed on days 0, 1, 3, 5, and 7. (A) Evaluation of PVR classification was performed with ultrasound echography as well as ophthalmoscopy. (B) Enucleated eyes were observed by stereomicroscopy. (A and B) A representative image of PVR with total retinal detachment (stage 5) in a rabbit injected with control IgG. (A and B) A representative image of PVR with fibrous tissue formation (arrow) and focal traction resulting in localized medullary ray detachment (stage 3) in a rabbit injected with periostin NAb.
 
Intravitreal injections of periostin NAb and control IgG were performed on days 0, 1, 3, 5, and 7. (A) Evaluation of PVR classification was performed with ultrasound echography as well as ophthalmoscopy. (B) Enucleated eyes were observed by stereomicroscopy. (A and B) A representative image of PVR with total retinal detachment (stage 5) in a rabbit injected with control IgG. (A and B) A representative image of PVR with fibrous tissue formation (arrow) and focal traction resulting in localized medullary ray detachment (stage 3) in a rabbit injected with periostin NAb.
 
Keywords: 655 proliferative vitreoretinopathy • 519 extracellular matrix • 765 wound healing  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×