June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Variations in Retinal Nuclear Areas in a Neonatal Mouse Model of Retinopathy of Prematurity by Histopathology
Author Affiliations & Notes
  • Olachi Mezu-Ndubuisi
    Pediatrics, University of Illinois at Chicago, Chicago, IL
  • Norman Blair
    Ophthalmology and Visual Sciences, University of Illinois, Chicago, IL
  • Amy Lin
    Pathology, University of Illinois, Chicago, IL
  • Justin Wanek
    Ophthalmology and Visual Sciences, University of Illinois, Chicago, IL
  • Narsa Reddy
    Pediatrics, University of Illinois at Chicago, Chicago, IL
  • Usha Raj
    Pediatrics, University of Illinois at Chicago, Chicago, IL
  • Sekhar Reddy
    Pediatrics, University of Illinois at Chicago, Chicago, IL
  • Mahnaz Shahidi
    Ophthalmology and Visual Sciences, University of Illinois, Chicago, IL
  • Footnotes
    Commercial Relationships Olachi Mezu-Ndubuisi, None; Norman Blair, None; Amy Lin, None; Justin Wanek, None; Narsa Reddy, None; Usha Raj, None; Sekhar Reddy, None; Mahnaz Shahidi, Patent (P)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 629. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Olachi Mezu-Ndubuisi, Norman Blair, Amy Lin, Justin Wanek, Narsa Reddy, Usha Raj, Sekhar Reddy, Mahnaz Shahidi; Variations in Retinal Nuclear Areas in a Neonatal Mouse Model of Retinopathy of Prematurity by Histopathology. Invest. Ophthalmol. Vis. Sci. 2013;54(15):629. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: Premature infants requiring supplemental oxygen are at risk of developing retinopathy of prematurity (ROP) and retinal structural and vascular abnormalities. The purpose of the current study is to investigate changes in retinal cell density in an oxygen-induced retinopathy (OIR) mouse model of ROP by quantitative analysis of ex-vivo histological slides.

Methods: Histological slides of the retina were obtained from enucleated eyes of OIR C57BL/6J mice (N = 6 eyes) and control mice (N = 6). OIR mice were exposed to 75% oxygen from post-natal day 7 (P7) to P12 before returning to room air, while control mice were exposed to room air continuously. Mice were sacrificed at P17-P19, the eyes enucleated, and histological slides stained with hematoxylin and eosin. The areas of the cell nuclei in the retinal inner nuclear and ganglion cell layers (inner retinal area) and in the retinal outer nuclear (outer retinal area) were quantified using a semi-automated morphometric image analysis software (Motic). These areas were divided by the total area of a selected region of the retina.

Results: The relative inner retinal area in OIR mice (14 ± 1%) and control mice (15 ± 1%) were similar (p = 0.2). Likewise, there was not a statistically significant difference in the outer retinal area in OIR mice (26 ± 2%) and control mice (23 ± 4%) (p = 0.2). In both control and OIR mice, the outer retinal area was significantly greater than the inner retinal area (p < 0.001). The sum of the inner and outer retinal areas in OIR mice (40 ± 2%) and control mice (38 ± 5%) was similar (p = 0.4). The ratio of the outer to inner retinal areas was significantly higher in OIR mice (1.9 ± 0.1) than control mice (1.6 ± 0.3) (p = 0.04).

Conclusions: In OIR mice, there was an increased proportion of outer to inner retinal nuclei, as compared to control mice. This may reflect variations in vascular supply and metabolic needs. Study of retinal structural changes may aid the understanding of ROP pathogenesis.

Keywords: 688 retina • 706 retinopathy of prematurity • 698 retinal development  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×