June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Protective Effects of Renin Inhibitor in Retinal Ischemia-reperfusion Injury
Author Affiliations & Notes
  • Kazuyuki Hirooka
    Ophthalmology, Kagawa Univ Faculty of Medicine, Kita-gun, Japan
  • Kaori Tenkumo
    Ophthalmology, Kagawa Univ Faculty of Medicine, Kita-gun, Japan
  • Eri Nitta
    Ophthalmology, Kagawa Univ Faculty of Medicine, Kita-gun, Japan
  • Tomoyoshi Fujita
    Ophthalmology, Kagawa Univ Faculty of Medicine, Kita-gun, Japan
  • Fumio Shiraga
    Ophthalmology, Kagawa Univ Faculty of Medicine, Kita-gun, Japan
  • Footnotes
    Commercial Relationships Kazuyuki Hirooka, None; Kaori Tenkumo, None; Eri Nitta, None; Tomoyoshi Fujita, None; Fumio Shiraga, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 6356. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Kazuyuki Hirooka, Kaori Tenkumo, Eri Nitta, Tomoyoshi Fujita, Fumio Shiraga; Protective Effects of Renin Inhibitor in Retinal Ischemia-reperfusion Injury. Invest. Ophthalmol. Vis. Sci. 2013;54(15):6356. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To investigate the effect of a renin inhibitor against retinal ischemia-reperfusion injury.

Methods: Retinal ischemia was induced by increasing intraocular pressure to 130 mmHg for 45 min. Rats were treated with a renin inhibitor (aliskiren). 25 mg/kg/day aliskiren or vehicle was administered by subcutaneous osmotic minipump 1 day before ischemia. At 7 days after ischemia, retinal damage was evaluated by measuring the retinal thickness and the number of retinal ganglion cells. Western blot was used to measure changes in the (pro)renin receptor expression. Data were analyzed using an independent Student’s t-test or Dunnett’s multiple comparison test.

Results: Retinal ganglion cell survival rates in the retinas with ischemia were 47.0% ± 5.7% in the vehicle-treated group and 102.3% ± 4.3% in the aliskiren-treated group (P < 0.001). In animals pretreated with distilled water, inner plexiform layer (IPL) and inner nuclear layer (INL) thicknesses were reduced to 64.8% ± 2.9% and 79.0% ± 3.3% of the control, respectively. When animals were pretreated with aliskiren, the IPL and INL thicknesses were 96.3% ± 1.0% (P < 0.001) and 102.3% ± 1.5% (P < 0.001) of the control, respectively. Although expression of (pro)renin receptor in the retina was similar between samples for the controls and after the reperfusion, (pro)renin receptor expression was decreased after reperfusion in the aliskiren-treated group.

Conclusions: Aliskiren may protect neurons by decreasing the expression of (pro)renin receptor after ischemia-reperfusion.

Keywords: 572 ischemia • 615 neuroprotection  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×