June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
MicroRNAs in vitreous humor from patients with ocular disease : preliminary results
Author Affiliations & Notes
  • Caterina Gagliano
    Ophthalmology, NEST (Neurovisual Science Technology), Catania, Italy
  • Andrea Russo
    Opthalmology, University of Catania, Catania, Italy
  • Marco Ragusa
    Molecular Biology, University of Catania, Catania, Italy
  • Rosario Caltabiano
    Pathological Anatomy, University of Catania, Catania, Italy
  • Lidia Puzzo
    Pathological Anatomy, University of Catania, Catania, Italy
  • Teresio Avitabile
    Opthalmology, University of Catania, Catania, Italy
  • Antonio Longo
    Opthalmology, University of Catania, Catania, Italy
  • Mario Toro
    Opthalmology, University of Catania, Catania, Italy
  • Michele Purrello
    Molecular Biology, University of Catania, Catania, Italy
  • Michele Reibaldi
    Opthalmology, University of Catania, Catania, Italy
  • Footnotes
    Commercial Relationships Caterina Gagliano, None; Andrea Russo, None; Marco Ragusa, None; Rosario Caltabiano, None; Lidia Puzzo, None; Teresio Avitabile, None; Antonio Longo, None; Mario Toro, None; Michele Purrello, None; Michele Reibaldi, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 6365. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Caterina Gagliano, Andrea Russo, Marco Ragusa, Rosario Caltabiano, Lidia Puzzo, Teresio Avitabile, Antonio Longo, Mario Toro, Michele Purrello, Michele Reibaldi; MicroRNAs in vitreous humor from patients with ocular disease : preliminary results. Invest. Ophthalmol. Vis. Sci. 2013;54(15):6365. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: Based on evidence that microRNAs (miRNAs) were found in many biological fluids (e.g., urine, saliva, pleural fluid), we sought to detect their presence and analyse their profile in vitreous humor (VH) from patients affected by different ocular diseases.

Methods: miRNAs were purified from VH samples taken during vitrectomy, by using Qiagen miRNeasy Mini Kit. The expression profile on 745 miRNAs was carried out by using TaqMan Low Density Array. Single TaqMan expression assays was performed on 18 VH samples (six each from patients with choroidal melanomas, retinal detachment, or macular hole, respectively), for miRNAs commonly expressed in serum or retinal cells: let-7b, mir-21, mir-26a, mir-146a, miR-199-3p, mir-210, mir-374a*, mir-532-5p. RNA extracted from serum of six healthy donors or from formalin-fixed, paraffin-embedded samples of choroidal melanocytes from four uveal melanomas (epithelioid cells) and from three unaffected eyes were used as controls.

Results: We have identified the presence of 94 circulating small RNAs in vitreous, some of which (miR-9, miR-9*, miR-125a-3p, miR-184, miR-211, miR-214, miR-302c, miR-452, miR-628, miR-639) are particularly abundant in VH but down-represented or not detectable in serum. Mir-146a and mir-26a were overexpressed more than three folds in VH from uveal melanoma patients with respect to the other pathological groups (Wilcoxon signed-rank test, p-value <0.05).

Conclusions: Our experimental data suggest that a specific set of circulating miRNAs is secreted in vitreous, which is quite different than miRNA pattern in serum, and that the quantity of vitreal miRNAs could change, depending on the pathologies of the eye.

Keywords: 763 vitreous  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×