June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
The Efficacy of Seprafilm® Graft with Adhesiolysis in Experimentally Induced Lid Adhesion in Rabbits
Author Affiliations & Notes
  • Hyemi Jung
    Department of Ophthalmology, Dong-A University College of Medicine, Busan, Republic of Korea
  • Hee-Bae Ahn
    Department of Ophthalmology, Dong-A University College of Medicine, Busan, Republic of Korea
  • Mee sook Roh
    pathology, Dong-A University College of Medicine, Busan, Republic of Korea
  • Won Yeol Ryu
    Department of Ophthalmology, Dong-A University College of Medicine, Busan, Republic of Korea
  • Yoon Hyung Kwon
    Department of Ophthalmology, Dong-A University College of Medicine, Busan, Republic of Korea
  • Footnotes
    Commercial Relationships Hyemi Jung, None; Hee-Bae Ahn, None; Mee sook Roh, None; Won Yeol Ryu, None; Yoon Hyung Kwon, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 6382. doi:https://doi.org/
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Hyemi Jung, Hee-Bae Ahn, Mee sook Roh, Won Yeol Ryu, Yoon Hyung Kwon; The Efficacy of Seprafilm® Graft with Adhesiolysis in Experimentally Induced Lid Adhesion in Rabbits. Invest. Ophthalmol. Vis. Sci. 2013;54(15):6382. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: To evaluate the efficacy of subsequent seprafilm® transplantation following adhesiolysis for preventing postoperative readhesion and improving the results of surgery.

Methods: Primary blepharoplasty was done on both eyelids of 18 albino rabbits. After 2 weeks, a new skin incision was made and adhesiolysis was done on both eyelids. The rabbits were categorized into two groups, one with adhesiolysis alone in left eyelids (control group), and the other with adhesiolysis with seprafilm® graft in right eyelids (seprafilm® group). The degree of inflammation and fibrosis were examined on Hematoxylin-eosin(H & E), Masson trichrome stain. Expression of α-smooth muscle actin(α-SMA) was immunohistochemically examined.

Results: On eyelid examination directly after the operation, mild swelling and hemorrhage was detected in both groups, but after 1 -2 week, the swelling and hemorrhage was resolved and the shape of the eyelids had recovered completely in both groups. In microscopic findings, the seprafilm® group showed less inflammation and fibrosis than the control group. The seprafilm® group showed less α -SMA positive cell than the conrol group.

Conclusions: From these findings, we suggest that the use of seprafilm® graft after adhesiolysis and blepharoplasty is an effective method for prevention of postoperative readhesion and good result of post blepharoplasty.

Keywords: 526 eyelid • 637 pathology: experimental  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×