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Tomoko Hasegawa, Hanako Ikeda, Noriko Nakano, Yuki Muraoka, Nagahisa Yoshimura; Optical coherence tomography, electroretinography, and histological analysis of mouse models for retinal degeneration. Invest. Ophthalmol. Vis. Sci. 2013;54(15):727. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To examine the natural course of retinal degeneration in mouse models
We performed optical coherence tomography (OCT) and dark-adapted electroretinography (ERG) examinations for 16 rd10 mice (32 eyes) aged 21 to 33 days, 25 rd12 mice (49 eyes) aged 13 months, and 10 rd12 mice (19 eyes) aged 19 months. Using the OCT images, we measured the thickness of the outer nuclear layer (ONL) and entire retinal thickness at a point 366 μm around the optic nerve head. We also performed electron microscopic examinations of the retina of a 21-day-old rd10 mouse and observed hematoxylin-eosin (HE)-stained retinal sections of an rd12 mouse.
The total retinal thickness and the ONL thickness of the rd10 mice were 153.4 ± 8.7 and 12.0 ± 3.8 μm (mean ± SD), respectively, at 25 days, and 140.4 ± 5.7 and 8.8 ± 1.5 μm, respectively, at 33 days. ERG showed diminished amplitude at 25 days and non-recordable results at 33 days. For rd12 mice, the total and ONL thickness were 205.2 ± 11.1 and 37.5 ± 4.4 μm, respectively, at 13 months and 198.2 ± 10.0 and 31.4 ± 3.7 μm, respectively, at 19 months. ERG showed non-recordable results at 13 months. OCT images of rd10 mice showed hyper-reflection from the ONL to the layer corresponding to the outer segment of the photoreceptors (OS) while the OCT images of rd12 mice showed hyper-reflection at the OS. The electron microscopic images of rd10 mice showed degeneration and disarrangement between the ONL and OS. Histological observation of rd12 mice showed thinning of the ONL and disarrangement of the OS.
The retinal thickness showed a substantial decrease in rd10 mice, but it showed only a moderate decrease in rd12 mice. While the ERG results were non-recordable in 13-month-old rd12 mice whose retinal thickness was relatively preserved, they were recordable in 25-day-old rd10 mice whose retina was quite thin. Hyper-reflection of the outer retina in OCT images may be caused by the disarrangement of the outer retina in histological observation.
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