June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Correlations between Lamina Cribrosa Microarchitecture and Thickness in Normal Non-Human Primate (NHP) Eyes
Author Affiliations & Notes
  • Howard Lockwood
    Discoveries in Sight Research Laboratories, Devers Eye Institute, Legacy Research Institute, Portland, OR
  • Juan Reynaud
    Discoveries in Sight Research Laboratories, Devers Eye Institute, Legacy Research Institute, Portland, OR
  • Hongli Yang
    Discoveries in Sight Research Laboratories, Devers Eye Institute, Legacy Research Institute, Portland, OR
  • Stuart Gardiner
    Discoveries in Sight Research Laboratories, Devers Eye Institute, Legacy Research Institute, Portland, OR
  • J Crawford Downs
    Ophthalmology, University of Alabama at Birmingham, Birmingham, AL
  • Claude Burgoyne
    Discoveries in Sight Research Laboratories, Devers Eye Institute, Legacy Research Institute, Portland, OR
  • Footnotes
    Commercial Relationships Howard Lockwood, None; Juan Reynaud, None; Hongli Yang, None; Stuart Gardiner, Allergan (R); J Crawford Downs, None; Claude Burgoyne, Heidelberg Engineering (F), Heidelberg Engineering (C)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 73. doi:
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    • Get Citation

      Howard Lockwood, Juan Reynaud, Hongli Yang, Stuart Gardiner, J Crawford Downs, Claude Burgoyne; Correlations between Lamina Cribrosa Microarchitecture and Thickness in Normal Non-Human Primate (NHP) Eyes. Invest. Ophthalmol. Vis. Sci. 2013;54(15):73.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To calculate and characterize the regional distributions of lamina cribrosa (LC) beam diameter (BD), pore diameter (PD), connective tissue volume (CTV) and connective tissue volume fraction (CTVF) from high-resolution, digital 3D optic nerve head (ONH) reconstructions of 23 normal NHP eyes so as to identify their correlations to LC thickness.

 
Methods
 

ONH reconstructions of 23 eyes (age 1.4 - 32.2 yrs) were aligned to a clinical photograph or confocal scanning laser tomographic (CSLT) image and the lamina cribrosa was 3D segmented [IEEE Trans Med Imag, 2006; 25:245-255]. Each voxel was assigned a “diameter” value based upon the largest sphere that fit inside the “beam” or “pore” in which it was contained. CTV was defined to be the total volume of beam voxels. CTVF was defined to be the ratio of CTV to total volume (pore + beam voxels). ONH, BD, PD, CTV and CTVF data were transformed into right eye orientation and mapped to a cylinder of radius 1.5 mm and 120 µm thickness. Data were regionalized into 12 sectors using the BMO centroid to foveal (FoBMO) axis as the horizontal midline allowing direct comparisons between eyes with varying laminar and scleral geometries. Mean BD and PD within each sector were normalized by dividing them by the standard deviation to ensure homogeneity of the residuals and hence validity of the subsequent analysis. Mixed effects models were constructed to detect effects of LC thickness accounting for spatially correlated data within each eye.

 
Results
 

BD (Mean / SD), CTV and CTVF were all significantly predicted by LC thickness; p=0.005 for BD, p<0.001 for CTV, and p=0.043 for CTVF. No relation was found between LC thickness and PD, p=0.149.

 
Conclusions
 

These data expand our previous characterizations of normal NHP LMA [H. Lockwood et al, ARVO, 2012; Roberts, et al IOVS. 2009;50:681-690] to include laminar BD, PD, CTV and CTVF to a total of 23 normal eyes. The regional distributions of normal NHP laminar BD, PD, CTV and CTVF are eye-specific, however pooled overall data for all 23 eyes (see plots below) suggest that BD and CTVF are correlated to LC thickness.

 
 
Upper plot shows Regional BD averages within 12 sectors. Lower plot shows BD correlation to lamina thickness. All regional data in OD configuration.
 
Upper plot shows Regional BD averages within 12 sectors. Lower plot shows BD correlation to lamina thickness. All regional data in OD configuration.
 
 
Upper plot shows Regional CTVF averages within 12 sectors. Lower plot shows CTVF correlation to lamina thickness. All regional data in OD configuration.
 
Upper plot shows Regional CTVF averages within 12 sectors. Lower plot shows CTVF correlation to lamina thickness. All regional data in OD configuration.
 
Keywords: 551 imaging/image analysis: non-clinical • 577 lamina cribrosa • 629 optic nerve  
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