June 2013
Volume 54, Issue 15
Free
ARVO Annual Meeting Abstract  |   June 2013
Pharmacological Actions of L-cysteine on Isolated Bovine Ciliary Muscles
Author Affiliations & Notes
  • Sunny Ohia
    Pharmaceutical Sciences, Texas Southern University, Houston, TX
  • Anita Okpobiri
    Pharmaceutical Sciences, Texas Southern University, Houston, TX
  • Ya Fatou Njie-Mbye
    Pharmaceutical Sciences, Texas Southern University, Houston, TX
  • Jenaye Robinson
    Pharmaceutical Sciences, Texas Southern University, Houston, TX
  • Madhura Chitnis
    Pharmaceutical Sciences, Texas Southern University, Houston, TX
  • Catherine Opere
    Department of Pharmacy Sciences, Creighton University, Omaha, NE
  • Footnotes
    Commercial Relationships Sunny Ohia, None; Anita Okpobiri, None; Ya Fatou Njie-Mbye, None; Jenaye Robinson, None; Madhura Chitnis, None; Catherine Opere, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2013, Vol.54, 787. doi:https://doi.org/
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      Sunny Ohia, Anita Okpobiri, Ya Fatou Njie-Mbye, Jenaye Robinson, Madhura Chitnis, Catherine Opere; Pharmacological Actions of L-cysteine on Isolated Bovine Ciliary Muscles. Invest. Ophthalmol. Vis. Sci. 2013;54(15):787. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: We have evidence that hydrogen sulfide ( H2S) donors such as sodium hydrosulfide (NaHS) can produce pharmacological actions on isolated porcine ocular smooth muscles. In this study, we investigated the pharmacological effects of L-cysteine, a substrate for H2S production, on isolated pre-contracted bovine ciliary muscle.

Methods: Isolated bovine ciliary muscle strips were set up in organ baths containing oxygenated Krebs buffer (pH 7.4) at 37°C. Changes in longitudinal isometric tension were recorded via grass FT03 Force-displacement transducers and analyzed using PolyView software. Effects of L-cysteine on carbachol-induced tone was studied in the presence of enzyme inhibitors for H2S biosynthetic pathway (AOA; cystathionine β-synthase inhibitor) and prostanoids (flurbiprofen; cyclo-oxygenase (COX) inhibitor).

Results: L-cysteine (0.01 nM-100 µM) evoked a concentration-dependent relaxation of carbachol-induced tone in bovine ciliary muscle, reaching a maximum inhibition of 88% at 10 µM (with an IC50 of 2 nM). Relaxations elicited by L-cysteine were unaffected by pre-treatment of tissues with flurbiprofen (3 μM). The inhibitor of cystathionine β-synthase, AOA (10 µM) blocked only relaxations caused by high concentrations of L-cysteine (> 500 nM).

Conclusions: L-cysteine can relax pre-contracted bovine ciliary muscle, and this effect is dependent on endogenous production of H2S. Furthermore, prostanoids are not involved in the inhibitory action of L-cysteine in this smooth muscle.

Keywords: 456 ciliary muscle • 616 neurotransmitters/neurotransmitter systems • 514 enzymes/enzyme inhibitors  
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