October 1971
Volume 10, Issue 10
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Articles  |   October 1971
Antibody in Tears Following Intranasal Vaccination With Inactivated Virus
Author Affiliations
  • HARRY L. S. KNOPF
    Laboratory of Vision Research, National Eye Institute, National Institute of Allergy and Infectious Diseases, and National Cancer Institute, National Institutes of Health, United States Department of Health, Education, and Welfare, Bethesda, Md. 20014
  • NEIL R. BLACKLOW
    Laboratory of Vision Research, National Eye Institute, National Institute of Allergy and Infectious Diseases, and National Cancer Institute, National Institutes of Health, United States Department of Health, Education, and Welfare, Bethesda, Md. 20014
  • MORRIS I. GLASSMAN
    Laboratory of Vision Research, National Eye Institute, National Institute of Allergy and Infectious Diseases, and National Cancer Institute, National Institutes of Health, United States Department of Health, Education, and Welfare, Bethesda, Md. 20014
  • WALTER L. CLINE
    Laboratory of Vision Research, National Eye Institute, National Institute of Allergy and Infectious Diseases, and National Cancer Institute, National Institutes of Health, United States Department of Health, Education, and Welfare, Bethesda, Md. 20014
  • VERNON G. WONG
    Laboratory of Vision Research, National Eye Institute, National Institute of Allergy and Infectious Diseases, and National Cancer Institute, National Institutes of Health, United States Department of Health, Education, and Welfare, Bethesda, Md. 20014
Investigative Ophthalmology & Visual Science October 1971, Vol.10, 750-759. doi:
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      HARRY L. S. KNOPF, NEIL R. BLACKLOW, MORRIS I. GLASSMAN, WALTER L. CLINE, VERNON G. WONG; Antibody in Tears Following Intranasal Vaccination With Inactivated Virus . Invest. Ophthalmol. Vis. Sci. 1971;10(10):750-759.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Rabbits were immunized with a formalin-treated vaccinia virus vaccine by means of intranasal or intradermal administration. A comparison was made between animals receiving vaccine with or without the adjuvant poly I:C. Two additional animals received live virus intradermally and were used as controls. The immunoglobulins of the tears and serum were analyzed, and it was found that: (1) IgA (IIS type) was the predominant immunoglobulin in the tears. (2) IgA appeared to be correlated with the antibody activity in tears of intranasally vaccinated animals. (3) The antiviral activity in the tears of intradermally vaccinated animals appeared to be IgG. (4) Poly I:C enhanced both the serum and tear antibody liters. (5) The predominant immunoglobulin and antibody in the serum was IgG. The indirect fluorescent antibody procedure was found to be a useful adjunctive method for assaying tear antibody. The possible mechanisms involved in the induction of tear antibodies and the effects of adjuvants are discussed.

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