Antibody in Tears Following Intranasal Vaccination With Inactivated Virus II. Enhancement of Tear Antibody Production by the Use of Polyinosinic: Polycytidilic Acid (Poly I:C)

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Abstract

Rabbits were immunized with a formalin-treated vaccinia virus vaccine by means of intranasal or intradermal administration. A comparison was made between animals receiving vaccine with or without the adjuvant poly I:C. Two additional animals received live virus intradermally and were used as controls. The immunoglobulins of the tears and serum were analyzed, and it was found that: (1) IgA (IIS type) was the predominant immunoglobulin in the tears. (2) IgA appeared to be correlated with the antibody activity in tears of intranasally vaccinated animals. (3) The antiviral activity in the tears of intradermally vaccinated animals appeared to be IgG. (4) Poly I:C enhanced both the serum and tear antibody liters. (5) The predominant immunoglobulin and antibody in the serum was IgG. The indirect fluorescent antibody procedure was found to be a useful adjunctive method for assaying tear antibody. The possible mechanisms involved in the induction of tear antibodies and the effects of adjuvants are discussed.