July 1971
Volume 10, Issue 7
Free
Articles  |   July 1971
Chorioretinal Diffusion of Peroxidase Before and After Photocoagulation
Author Affiliations
  • GHOLAM A. PEYMAN
    Department of Ophthalmology and Jules Stein Eye Institute, UCLA School of Medicine Los Angeles, Calif. 90024.
  • MANFRED SPITZNAS
    Department of Ophthalmology and Jules Stein Eye Institute, UCLA School of Medicine Los Angeles, Calif. 90024.
  • BRADLEY R. STRAATSMA
    Department of Ophthalmology and Jules Stein Eye Institute, UCLA School of Medicine Los Angeles, Calif. 90024.
Investigative Ophthalmology & Visual Science July 1971, Vol.10, 489-495. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      GHOLAM A. PEYMAN, MANFRED SPITZNAS, BRADLEY R. STRAATSMA; Chorioretinal Diffusion of Peroxidase Before and After Photocoagulation. Invest. Ophthalmol. Vis. Sci. 1971;10(7):489-495.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

To study chorioretinal diffusion mechanisms in the mammalian retina, the peroxidase tracer technique, electron microscopy, and photocoagulation were used in a series of Dutch rabbits. Xenon arc photocoagulation of the retina was carried out on one eye of each animal, and three weeks later peroxidase was injected into the ear vein. At selected time intervals thereafter, the animals were killed, and the eyes were processed for electron microscopy. In the nonphotocoagulated eyes, tracer material diffused rapidly from the choriocapillaris, passed through Bruch's membrane, filled the basal infoldings of the pigment epithelium, and extended into the intercellular space between the pigment epithelium cells until it was stopped at the external margin of the zonulae occludentes. In the photocoagulated retina, peroxidase passed from the choroid, extended through Bruch's membrane, and diffused through the intercellular spaces of the external scar cells. Although there was no evidence of a zonula occludens-type junction, no tracer material was observed close to or central to the internal limiting membrane during this study. Consideration is given to the physiologic implications of these observations and to the mechanisms of action of photocoagulation in certan retinal diseases.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×