Purchase this article with an account.
GHOLAM A. PEYMAN, MANFRED SPITZNAS, BRADLEY R. STRAATSMA; Chorioretinal Diffusion of Peroxidase Before and After Photocoagulation. Invest. Ophthalmol. Vis. Sci. 1971;10(7):489-495.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To study chorioretinal diffusion mechanisms in the mammalian retina, the peroxidase tracer technique, electron microscopy, and photocoagulation were used in a series of Dutch rabbits. Xenon arc photocoagulation of the retina was carried out on one eye of each animal, and three weeks later peroxidase was injected into the ear vein. At selected time intervals thereafter, the animals were killed, and the eyes were processed for electron microscopy. In the nonphotocoagulated eyes, tracer material diffused rapidly from the choriocapillaris, passed through Bruch's membrane, filled the basal infoldings of the pigment epithelium, and extended into the intercellular space between the pigment epithelium cells until it was stopped at the external margin of the zonulae occludentes. In the photocoagulated retina, peroxidase passed from the choroid, extended through Bruch's membrane, and diffused through the intercellular spaces of the external scar cells. Although there was no evidence of a zonula occludens-type junction, no tracer material was observed close to or central to the internal limiting membrane during this study. Consideration is given to the physiologic implications of these observations and to the mechanisms of action of photocoagulation in certan retinal diseases.
This PDF is available to Subscribers Only