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D Benezra, U Sachs; Growth and transplantation of organ-cultured corneas.. Invest. Ophthalmol. Vis. Sci. 1975;14(1):24-26.
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Calf corneal buttons were grown in medium M199 supplemented with newborn calf serum (xenogeneic to the recipient) or rabbit serum (allongeneic to the recipient) and implanted interlamellarly into rabbit recipients. Implantation of buttons, grown in medium containing calf serum were rejected sooner than noncultured buttons. The rejection was quicker as the grafts were kept longer in culture. However, corneal buttons grown in medium containing rabbit serum showed, in general, a slower rejection process.
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