March 1974
Volume 13, Issue 3
Free
Articles  |   March 1974
Intermediate-Term Corneal Storage
Author Affiliations
  • TAKASHI SAKIMOTO
    Department of Ophthalmology, College of Medicine, University of Florida, Gainesville, Fla.
  • JOHN VALENTI
    National Eye Institute and an International Scholar's Award from Research to Prevent Blindness, Inc.
  • MOTOKAZU ITOI
    Department of Ophthalmology, College of Medicine, University of Florida, Gainesville, Fla.
  • HERBERT E. KAUFMAN
    Department of Ophthalmology, College of Medicine, University of Florida, Gainesville, Fla.
Investigative Ophthalmology & Visual Science March 1974, Vol.13, 219-228. doi:https://doi.org/
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      TAKASHI SAKIMOTO, JOHN VALENTI, MOTOKAZU ITOI, HERBERT E. KAUFMAN; Intermediate-Term Corneal Storage. Invest. Ophthalmol. Vis. Sci. 1974;13(3):219-228. doi: https://doi.org/.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
This content is PDF only. Please click on the PDF icon to access.
Abstract

Human and rabbit corneas were evaluated for suitability for keratoplasty donor material after storage in moist chambers or soaked in various media. A new chamber was designed which allows corneas to be stored for up to two weeks at ordinary refrigerator temperature. Corneas with scleral rims were placed in the chamber at 4° C. The epithelium faced down into the medium of modified Ringer's or physiologic saline plus Dextran-40 while the endothelium faced a moist chamber. The chamber was then placed in the refrigerator and the cornea sustained one or two weeks storage at 4° C. Corneas were evaluated by means of light and electron microscopy, specular microscopy, and penetrating keratoplasty. Of 12 grafts performed using rabbit corneas stored in this new chamber, 10 grafts were successful. Human corneas were also stored in the chamber, and compared favorably under electron microscopic observation with the mate control corneas.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×