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D J Spence, G A Peyman; A new technique for the vital staining of the corneal endothelium.. Invest. Ophthalmol. Vis. Sci. 1976;15(12):1000-1002.
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A new technique for the staining of the corneal endothelium combines the actions of the vital stain trypan blue and the intercellular stain alizarin red S. The technique is an improvement over former staining methods, because it defines both viable and nonviable cells and thereby permits an investigator to accurately quantify endothelial cell damage.
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