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R F Meyer, B E McCarey, J Valenti, N Gravenstein, H E Kaufman; Scanning electron microscopy of postoperative M-K and moist chamber-stored corneas.. Invest. Ophthalmol. Vis. Sci. 1976;15(4):260-266. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
In the present study, using the in vivo test situation which all corneal donor material must meet, we performed penetrating keratoplastics utilizing corneal tissue stored for five days in either M-K medium or conventional moist chambers. Cat corneas stored for five days in M-K medium had a higher incidence of postoperative graft clarity along with an intact endothelial cell layer as determined by scanning electron microscopy than corneas stored in a conventional moist chamber. The endothelium of corneas immediately after moist chamber storage revealed minimal surface alteration which contrasted to the more severely stressed endothelium immediately after M-K storage. However, the M-K stored endothelium survived the trauma of penetrating keratoplasty much better than cells stored in moist chambers.
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