April 1976
Volume 15, Issue 4
Articles  |   April 1976
Scanning electron microscopy of postoperative M-K and moist chamber-stored corneas.
Investigative Ophthalmology & Visual Science April 1976, Vol.15, 260-266. doi:
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      R F Meyer, B E McCarey, J Valenti, N Gravenstein, H E Kaufman; Scanning electron microscopy of postoperative M-K and moist chamber-stored corneas.. Invest. Ophthalmol. Vis. Sci. 1976;15(4):260-266.

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      © ARVO (1962-2015); The Authors (2016-present)

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In the present study, using the in vivo test situation which all corneal donor material must meet, we performed penetrating keratoplastics utilizing corneal tissue stored for five days in either M-K medium or conventional moist chambers. Cat corneas stored for five days in M-K medium had a higher incidence of postoperative graft clarity along with an intact endothelial cell layer as determined by scanning electron microscopy than corneas stored in a conventional moist chamber. The endothelium of corneas immediately after moist chamber storage revealed minimal surface alteration which contrasted to the more severely stressed endothelium immediately after M-K storage. However, the M-K stored endothelium survived the trauma of penetrating keratoplasty much better than cells stored in moist chambers.


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