January 1979
Volume 18, Issue 1
Articles  |   January 1979
Long-term perfusion of human corneas.
Investigative Ophthalmology & Visual Science January 1979, Vol.18, 107-111. doi:
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      B H Schimmelpfennig; Long-term perfusion of human corneas.. Invest. Ophthalmol. Vis. Sci. 1979;18(1):107-111.

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      © ARVO (1962-2015); The Authors (2016-present)

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In twelve isolated human corneas (cadaver time 2 to 16 hr) stromal thickness could be maintained within 0.50 to 0.66 mm up to 20 hr by the use of an endothelial bathing medium consisting of glutathione-bicarbonate Ringer's solution, HEPES-buffer, and 20% human serum. During the first 3 to 5 hr of perfusion, temperature reversal occurred in all corneas. Wound healing processes were observed in nine corneas throughout the experiments after scraping off a 2.5 mm area of central epithelium, which was kept in a moist saline-saturated atmosphere.


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