December 1980
Volume 19, Issue 12
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Articles  |   December 1980
Replication of acute hemorrhagic conjunctivitis viruses in conjunctival-corneal cell cultures of mice, rabbits, and monkeys.
Investigative Ophthalmology & Visual Science December 1980, Vol.19, 1477-1482. doi:
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      M P Langford, G J Stanton; Replication of acute hemorrhagic conjunctivitis viruses in conjunctival-corneal cell cultures of mice, rabbits, and monkeys.. Invest. Ophthalmol. Vis. Sci. 1980;19(12):1477-1482.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Acute hemorrhagic conjunctivitis (AHC) viruses--enterovirus type 70 (E70), and coxsackie-virus type A24 (CA24)--infect the superficial cells of the conjunctiva and cornea. We examined the growth of several E70 and CA24 isolates in monkey, rabbit, and mouse conjunctival and corneal (C/C) cells. We found that E70 isolates grew well in monkey (10(6.5) TCID50/ml), rabbit (10(4.5) to 10(5.5) TCID50/ml), and mouse (10(3.5) to 10(6.0) TCID50/ml) C/C cultures and caused cytopathic effects (CPE). In contrast, CA24 isolates replicated well and caused CPE only in monkey C/C cultures (10(6.5) TCID50/ml). These results show that E70 isolates grow readily in monkey, rabbit, and mouse C/C cells whereas CA24 isolates replicated in monkey C/C cells only. This finding is consistent with the suggestion that a lower animal reservoir may be a source of human infection by E70. These cell culture systems may be useful for studying virus-cell interactions and host defenses operating at the surface of the eye and for evaluating the feasibility of prophylactic or therapeutic use of interferon, interferon inducers, and/or antibody for controlling AHC virus infections.

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