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N A Schellart, R P Duursma; O2 consumption and CO2 influence on the pH and electroretinogram of isolated retina of goldfish.. Invest. Ophthalmol. Vis. Sci. 1980;19(9):1110-1113.
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From micropolarographic O2 measurements, it can be concluded that the isolated goldfish retina is sufficiently supplied with oxygen when 100% O2 is blown over the retina. The retinal O2 consumption amounts to about 60 microliters of O2 per milligram dry weight per hour. Small amounts (0.25% to 2.0%) of CO2 added to the O2 gas diminish the retinal pH, which is 7.0 to 7.1 in situ and after isolation. Moreover, CO2 strongly reduces the ERG amplitude.
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