December 1980
Volume 19, Issue 12
Free
Articles  |   December 1980
A simple staining method for blood vessels in flat preparations of ocular tissues.
Investigative Ophthalmology & Visual Science December 1980, Vol.19, 1518-1521. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      G Raviola, T F Freddo; A simple staining method for blood vessels in flat preparations of ocular tissues.. Invest. Ophthalmol. Vis. Sci. 1980;19(12):1518-1521.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

We describe a technique for light microscopic visualization of the vasculature of the retina, choroid, ciliary body, and iris in flat preparations. The technique is based on the intravenous injection of horseradish peroxidase, the histochemical demonstration of this enzyme's activity, and the bleaching of the melanin that hinders the visualization of the injected vessels. This technique is reproducible and much simpler than other available methods in which dyes suspended in different media are injected directly into the ocular vessels.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×