March 1980
Volume 19, Issue 3
Articles  |   March 1980
Characterization of retinoblastoma immune complexes.
Investigative Ophthalmology & Visual Science March 1980, Vol.19, 302-305. doi:
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      P C Stein, M Christensen, D H Char; Characterization of retinoblastoma immune complexes.. Invest. Ophthalmol. Vis. Sci. 1980;19(3):302-305.

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      © ARVO (1962-2015); The Authors (2016-present)

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Immune complexes from retinoblastoma sera were characterized with molecular sieve chromatography, affinity chromatography, and polyacrylamide gel electrophoresis (PAGE). Retinoblastoma patients' sera had two well-defined peaks of immune complex activity after molecular sieve chromatography. These protein fractions had a molecular weight of approximately 1.6 x 10(5) and 2.0 x 10(6) daltons. Affinity chromatography with Sepharose 4B-protein A and analytical PAGE demonstrated that IgG was the predominant immunoglobulin in these immune compelxes. Immune complexes also had affinity for Sepharose-concanavalin A, indicating the glycoprotein nature of the antigen component.


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