April 1981
Volume 20, Issue 4
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Articles  |   April 1981
Lacrimal gland flow and potentials during dinitrophenol, ouabain, and ethacrynic acid perfusion.
Investigative Ophthalmology & Visual Science April 1981, Vol.20, 515-521. doi:
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      S Y Botelho, N Fuenmayor; Lacrimal gland flow and potentials during dinitrophenol, ouabain, and ethacrynic acid perfusion.. Invest. Ophthalmol. Vis. Sci. 1981;20(4):515-521.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Acetylcholine (ACh)-evoked flow from the main excretory duct as well as ACh-induced secretory and resting membrane potentials from cells of the rabbit lacrimal gland were recorded during perfusion with inhibitors. During perfusion with 2,4-dinitrophenol (DNP), ouabain, or ethacrynic acid, ACh-induced flow was 5%, 20%, and 8% of control, respectively; ACh-induced hyperpolarizing secretory potential was 64%, 50%, and 63% of control, respectively; and the resting membrane potential was 79%, 64%, and 73% of control, respectively. Only during perfusion with ethacrynic acid was there a significant increase in the number of cells that did not respond to ACh with a change in potential. We have concluded that (1) ACh-induced secretion is highly dependent on oxidative metabolism and Na-K ATPase; (2) ACh-induced hyperpolarization is dependent on changes in ionic permeabilities, Na-K ATPase, and to lesser extent oxidative metabolism; and (3) the resting membrane potential is much less dependent on oxidative metabolism and Na-K ATPase activity.

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