January 1982
Volume 22, Issue 1
Articles  |   January 1982
Isolation and characterization of fibronectin from bovine aqueous humor.
Investigative Ophthalmology & Visual Science January 1982, Vol.22, 57-61. doi:
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      T Reid, C Kenney, G O Waring; Isolation and characterization of fibronectin from bovine aqueous humor.. Invest. Ophthalmol. Vis. Sci. 1982;22(1):57-61.

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      © ARVO (1962-2015); The Authors (2016-present)

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Aqueous humor collected from freshly enucleated bovine eyes was passed over a gelatin affinity column, and bound material was eluted with 4M urea. When fractionated by sodium dodecyl sulfate electrophoresis, the eluted material co-migrated with human plasma fibronectin and protein standards of approximately 220,000 daltons. Amino acid analyses demonstrated a composition similar to that of human plasma fibronectin and fibronectin synthesized by cultured cells. The concentration of fibronectin in aqueous humor is approximately 100 times less than in human plasma.


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