May 1984
Volume 25, Issue 5
Articles  |   May 1984
The distribution of HLA antigens on human corneal tissue.
Investigative Ophthalmology & Visual Science May 1984, Vol.25, 519-524. doi:
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      C F Whitsett, R D Stulting; The distribution of HLA antigens on human corneal tissue.. Invest. Ophthalmol. Vis. Sci. 1984;25(5):519-524.

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      © ARVO (1962-2015); The Authors (2016-present)

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Frozen sections of human corneas, as well as cultured cells derived from the epithelial, stromal, and endothelial layers, were examined for class I (HLA-A, B, C) and class II (HLA-DR) histocompatibility antigens using mouse monoclonal antibodies in an indirect immunofluorescence assay. On frozen sections, class I antigens were readily detected on corneal epithelium and keratocytes. Class I antigens were not detected on endothelial cells on frozen sections of adult corneas, but were identified on endothelial cells from some individuals less than 2 years of age. Class II antigens were not detected on corneal epithelial, stromal or endothelial cells on frozen sections. However, HLA-DR-positive dendritic cells were seen in corneal epithelium and were more numerous near the limbus. HLA-DR was expressed by cuboidal cells in the basal layer of conjunctival epithelium from several infants. Cultured cells derived from corneal epithelium, stroma, and endothelium consistently expressed class I but not class II antigens.


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