May 1984
Volume 25, Issue 5
Articles  |   May 1984
Fluorophors and chromophors from rat lens crystallins in UV with hydroxykynurenine.
Investigative Ophthalmology & Visual Science May 1984, Vol.25, 581-585. doi:
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      M Bando, N T Yu, J F Kuck; Fluorophors and chromophors from rat lens crystallins in UV with hydroxykynurenine.. Invest. Ophthalmol. Vis. Sci. 1984;25(5):581-585.

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      © ARVO (1962-2015); The Authors (2016-present)

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Isolated alpha-, beta-, and gamma-crystallins from young rat lenses were incubated in solution for 16 hr with 3-hydroxykynurenine under ultraviolet (366 nm) light. Controls included: incubation without light, without kynurenine, and with 2-mercaptoethanol. These procedures generated several chromophors (with absorption maxima or shoulders at 340, 370, and 470 nm) and fluorophors (with excitation/emission at 407/515, 458/550, 515/555, 647/664, and 647/740 nm). The formation of these pigments was inhibited by 2-mercaptoethanol. The findings are discussed in relation to the chromophors and fluorophors found in aged and brunescent human lenses.


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