April 1986
Volume 27, Issue 4
Free
Articles  |   April 1986
Rabbit corneal endothelial cells in vitro: effects of EGF.
Investigative Ophthalmology & Visual Science April 1986, Vol.27, 474-479. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      G M Raymond, M M Jumblatt, S P Bartels, A H Neufeld; Rabbit corneal endothelial cells in vitro: effects of EGF.. Invest. Ophthalmol. Vis. Sci. 1986;27(4):474-479.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Rabbit corneal endothelial cells were grown in tissue culture. Epidermal growth factor (EGF) increased the mitotic rate during the growth phase by 70% over control without affecting the plating efficiency. Within 48 hr of exposure to EGF, the endothelial cells became spindle-shaped. This morphological change was quantitated by morphometry; cells treated with EGF had a major axis 1.5 X larger than that of non-EGF treated cells. The spindle-shaped morphological change did not occur in response to other growth factors, was not related to cell density, and was reversible within 24 hr after removal of EGF from the media or subculture in the absence of EGF. The addition of 5-fluorouracil blocked cell division but did not affect the EGF-induced morphological change. The appearance of the endothelial cells following EGF stimulation is similar to migrating cells closing a wound in vivo.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×