April 1988
Volume 29, Issue 4
Free
Articles  |   April 1988
Pharmacological regulation of morphology and mitosis in cultured rabbit corneal endothelium.
Author Affiliations
  • M M Jumblatt
    Ophthalmic Pharmacology Unit, Eye Research Institute, Boston, MA 02114.
  • E D Matkin
    Ophthalmic Pharmacology Unit, Eye Research Institute, Boston, MA 02114.
  • A H Neufeld
    Ophthalmic Pharmacology Unit, Eye Research Institute, Boston, MA 02114.
Investigative Ophthalmology & Visual Science April 1988, Vol.29, 586-593. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M M Jumblatt, E D Matkin, A H Neufeld; Pharmacological regulation of morphology and mitosis in cultured rabbit corneal endothelium.. Invest. Ophthalmol. Vis. Sci. 1988;29(4):586-593.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Cultured rabbit corneal endothelial cells elongate when grown in the presence of epidermal growth factor (EGF) and indomethacin (INDO); whereas maintenance of the differentiated polygonal cell shape is apparently dependent upon endogenous synthesis of prostaglandin E2 (PGE2). In the current study, the authors demonstrate morphological changes in phenotypically altered cells and identify two intracellular pathways which interdependently regulate endothelial cells. Morphometric and mitotic analyses of cultures treated with a variety of pharmacological agents indicate that both protein kinases A- and C-dependent pathways regulate cell shape and cell division in corneal endothelial cells. Marked intracellular reorganization is associated with the morphological changes in the endothelial cells. When stained with rhodamine conjugated phallicidin, polygonal endothelial cells have circumferential bands of f-actin at their borders. EGF and/or INDO induce elongation and redistribution of f-actin into a diffuse cytoplasmic reticulum. Transmission electron microscopy demonstrates loss of several characteristic morphological markers for endothelial cells in response to pharmacologically induced elongation. The elongated cells lose intracellular junctions, apical/basal polarity and rough endoplasmic reticulum. These ultrastructural markers and circumferential f-actin bands are restored in cultures supplemented with exogenous PGE2. Modulation of these pathways in vivo may regulate cellular migration and mitosis during wound closure, stress, trauma and with age.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×