Purchase this article with an account.
S Z Abghari, R D Stulting, S M Nigida, D N Downer, A J Nahmias; Comparative replication of HSV-1 in BALB/c and C57BL/6 mouse embryo fibroblasts in vitro.. Invest. Ophthalmol. Vis. Sci. 1986;27(6):909-914.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Herpes simplex virus type 1 (HSV)-host cell interactions were studied in fibroblasts from inbred mice by measuring virus replication, virus adsorption, infectious center formation, and single-cell virus production. BALB/c mouse embryo fibroblasts (MEF) produced more intracellular and extracellular virus than C57BL/6 MEF, with differences in virus production first appearing at 16 hr after inoculation. Virus yield in C57BL/6 cells peaked earlier (16 hr) and at a lower level than in BALB/c cells (20 hr). These results were explained by a difference in single-cell virus replication, rather than less efficient adsorption or the presence of cells that could not be infected. Host-related variation in the ability of infected cells to support HSV replication may account, in part, for differences in the severity of HSV ocular disease.
This PDF is available to Subscribers Only