November 1987
Volume 28, Issue 11
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Articles  |   November 1987
Angiotensin binding sites in bovine and human retinal blood vessels.
Author Affiliations
  • G Ferrari-Dileo
    Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami School of Medicine, FL 33101.
  • E B Davis
    Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami School of Medicine, FL 33101.
  • D R Anderson
    Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami School of Medicine, FL 33101.
Investigative Ophthalmology & Visual Science November 1987, Vol.28, 1747-1751. doi:
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      G Ferrari-Dileo, E B Davis, D R Anderson; Angiotensin binding sites in bovine and human retinal blood vessels.. Invest. Ophthalmol. Vis. Sci. 1987;28(11):1747-1751.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Blood vessels isolated from bovine and human retinas have sites that specifically bind 3H-angiotensin II (3H-Ag II) with an apparent dissociation constant (Kd) of 14 nM and a capacity of binding (Bmax) of 0.82 pmol/g. The binding sites for 3H-Ag II appear to be influenced by guanine nucleotides (GTP) and cations (Mg2+ and Na+) in a way that resembles angiotensin II receptors in other tissues. The physiologic effect of blood-borne or locally-formed angiotensin II on retinal blood flow remains to be defined.

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