Purchase this article with an account.
L Takemoto, M Takehana, J Horwitz; Covalent changes in MIP26K during aging of the human lens membrane.. Invest. Ophthalmol. Vis. Sci. 1986;27(3):443-446.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Rabbit antisera have been made to the C-terminal octapeptide of bovine MIP26K (residues 256-263) and to an octapeptide near the C-terminus (residues 252-259). Use of these antisera in Western blot analysis of membrane from young vs old human lenses, and from cortex vs nucleus of old human lenses, suggest age-related covalent changes in the C-terminus of MIP26K. These age-related changes in anti-256-263 and anti-252-259 sera can be mimicked by treatment of lens membrane with carboxypeptidase Y, suggesting the loss of 1-8 amino acid residues from the C-terminus of the 26,000 dalton form of MIP26K during aging of the normal human lens.
This PDF is available to Subscribers Only