Use of immunotoxin to inhibit proliferating human corneal endothelium.

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Abstract

Transferrin plays a central role in cellular proliferation and proliferating cells have been shown to express transferrin receptors with increased density. We examined the effect of an immunotoxin consisting of anti-transferrin receptor monoclonal antibody (454A12) conjugated to recombinant ricin A chain (rRTA) on the proliferation of human corneal endothelium (HCE) in vitro. In proliferating cultures an immunotoxin (454A12-rRTA) concentration of 50 ng/mL reduced cell counts at day 7 by at least 89%, with no effect observed at 0.01 ng/ml. In contrast, cell counts were only minimally reduced in confluent cultures, even after 7 days' exposure to high concentrations of immunotoxin. These data suggest that 454A12-rRTA may be used to prevent growth of human corneal endothelium in pathological conditions such as the iridocorneal endothelial (ICE) syndrome.