May 1988
Volume 29, Issue 5
Free
Articles  |   May 1988
Use of immunotoxin to inhibit proliferating human corneal endothelium.
Author Affiliations
  • S Fulcher
    Scott and White Memorial Hospital, Department of Ophthalmology, Temple, TX 76508.
  • G M Lui
    Scott and White Memorial Hospital, Department of Ophthalmology, Temple, TX 76508.
  • L L Houston
    Scott and White Memorial Hospital, Department of Ophthalmology, Temple, TX 76508.
  • S Ramakrishnan
    Scott and White Memorial Hospital, Department of Ophthalmology, Temple, TX 76508.
  • T Burris
    Scott and White Memorial Hospital, Department of Ophthalmology, Temple, TX 76508.
  • J Polansky
    Scott and White Memorial Hospital, Department of Ophthalmology, Temple, TX 76508.
  • J Alvarado
    Scott and White Memorial Hospital, Department of Ophthalmology, Temple, TX 76508.
Investigative Ophthalmology & Visual Science May 1988, Vol.29, 755-759. doi:
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    • Get Citation

      S Fulcher, G M Lui, L L Houston, S Ramakrishnan, T Burris, J Polansky, J Alvarado; Use of immunotoxin to inhibit proliferating human corneal endothelium.. Invest. Ophthalmol. Vis. Sci. 1988;29(5):755-759.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Transferrin plays a central role in cellular proliferation and proliferating cells have been shown to express transferrin receptors with increased density. We examined the effect of an immunotoxin consisting of anti-transferrin receptor monoclonal antibody (454A12) conjugated to recombinant ricin A chain (rRTA) on the proliferation of human corneal endothelium (HCE) in vitro. In proliferating cultures an immunotoxin (454A12-rRTA) concentration of 50 ng/mL reduced cell counts at day 7 by at least 89%, with no effect observed at 0.01 ng/ml. In contrast, cell counts were only minimally reduced in confluent cultures, even after 7 days' exposure to high concentrations of immunotoxin. These data suggest that 454A12-rRTA may be used to prevent growth of human corneal endothelium in pathological conditions such as the iridocorneal endothelial (ICE) syndrome.

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