April 1988
Volume 29, Issue 4
Free
Articles  |   April 1988
Prediction of metastasis of uveal melanoma: comparison of morphometric determination of nucleolar size and spectrophotometric determination of DNA.
Author Affiliations
  • I W McLean
    Department of Ophthalmic Pathology, Armed Forces Institute of Pathology, Washington, DC 20306-6000.
  • J W Gamel
    Department of Ophthalmic Pathology, Armed Forces Institute of Pathology, Washington, DC 20306-6000.
Investigative Ophthalmology & Visual Science April 1988, Vol.29, 507-511. doi:
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      I W McLean, J W Gamel; Prediction of metastasis of uveal melanoma: comparison of morphometric determination of nucleolar size and spectrophotometric determination of DNA.. Invest. Ophthalmol. Vis. Sci. 1988;29(4):507-511.

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Abstract

We studied 95 cases of uveal melanoma using cytomorphometry to measure the standard deviation of nucleolar area (SD-NA) based on 200 cells per tumor and microspectrophotometry to determine the quantity of DNA in the nucleus of 100 cells per tumor. The 95 cases of uveal melanoma, in which the eye was enucleated between 1970 and 1973, were selected from the files of the REgistry of Ophthalmic Pathology. Forty-nine patients survived with a median follow-up of 15 years without evidence of metastasis at the time of the last follow-up, and 46 patients died of metastatic melanoma. Statistical analysis indicated that SD-NA was a better predictor of metastasis than DNA determination in this data set.

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