February 1987
Volume 28, Issue 2
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Articles  |   February 1987
Unscheduled DNA synthesis following excimer laser ablation of the cornea in vivo.
Author Affiliations
  • R C Nuss
    Laser Research Laboratory, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114, USA.
  • C A Puliafito
    Laser Research Laboratory, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114, USA.
  • E Dehm
    Laser Research Laboratory, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114, USA.
Investigative Ophthalmology & Visual Science February 1987, Vol.28, 287-294. doi:
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      R C Nuss, C A Puliafito, E Dehm; Unscheduled DNA synthesis following excimer laser ablation of the cornea in vivo.. Invest. Ophthalmol. Vis. Sci. 1987;28(2):287-294.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

The amount of unscheduled DNA synthesis (UDS) produced in the cells adjacent to excimer laser ablations in the cornea was compared for 193-nm and 248-nm laser wavelengths. UDS is interpreted to indicate the process of excision repair of pyrimidine dimers formed in DNA. 193-nm laser ablation did not produce a statistically significant difference in the amount of UDS as compared to a negative control (diamond-knife corneal incision). However, 248-nm laser ablation did produce a highly statistically significant difference in the amount of UDS as compared to both the negative control (P < 0.001) and the 193-nm laser irradiation (P < 0.001). Other forms of DNA damage (single-strand DNA chain breaks and DNA-protein crosslinks) are not measurable by UDS and need to be investigated in the evaluation of the oncogenic potential of 193-nm laser ablation.

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