May 1988
Volume 29, Issue 5
Free
Articles  |   May 1988
Isolation of non-pigmented epithelial cells from rabbit ciliary body.
Author Affiliations
  • G L Fain
    Department of Ophthalmology, Jules Stein Eye Institute, UCLA School of Medicine 90024-1771.
  • M C Cilluffo
    Department of Ophthalmology, Jules Stein Eye Institute, UCLA School of Medicine 90024-1771.
  • M J Fain
    Department of Ophthalmology, Jules Stein Eye Institute, UCLA School of Medicine 90024-1771.
  • D A Lee
    Department of Ophthalmology, Jules Stein Eye Institute, UCLA School of Medicine 90024-1771.
Investigative Ophthalmology & Visual Science May 1988, Vol.29, 817-821. doi:
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    • Get Citation

      G L Fain, M C Cilluffo, M J Fain, D A Lee; Isolation of non-pigmented epithelial cells from rabbit ciliary body.. Invest. Ophthalmol. Vis. Sci. 1988;29(5):817-821.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

We describe a new technique for the separation and isolation of nonpigmented ciliary body epithelial cells from rabbit. Excised ciliary body is incubated in a medium buffered with EGTA to a free-Ca2+ concentration of 10(-8) M, and the nonpigmented cell layer is separated from the pigmented layer by microdissection under direct visualization. This technique yields intact cells which are greater than 99% nonpigmented. It can be used to produce preparations of nonpigmented cell plasma membrane and of viable whole cells, which may be useful for biochemistry, pharmacology, cell transport studies and tissue culture.

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