May 1988
Volume 29, Issue 5
Free
Articles  |   May 1988
High activities of NADP+-dependent isocitrate dehydrogenase and malic enzyme in rabbit lens epithelial cells.
Author Affiliations
  • B S Winkler
    Eye Research Institute of Oakland University, Rochester, MI 48309.
  • F Solomon
    Eye Research Institute of Oakland University, Rochester, MI 48309.
Investigative Ophthalmology & Visual Science May 1988, Vol.29, 821-823. doi:
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      B S Winkler, F Solomon; High activities of NADP+-dependent isocitrate dehydrogenase and malic enzyme in rabbit lens epithelial cells.. Invest. Ophthalmol. Vis. Sci. 1988;29(5):821-823.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Measurements were made of the activities of the NADP+-dependent isocitrate dehydrogenase, malic enzyme and glucose-6-phosphate dehydrogenase in cytosolic supernatants of whole lens and capsule-epithelium. The activities of all three NADP+-dependent enzymes were concentrated in the capsule-epithelium relative to the activities measured in the whole lens. These results show for the first time that the rabbit lens epithelial cells contain substantial activities of the NADP+-dependent isocitrate dehydrogenase and malic enzyme. It is proposed that these enzymes together with glucose-6-phosphate dehydrogenase are involved in the regulation of the intracellular level of glutathione during oxidative stress by providing the NADPH necessary for the activity of glutathione reductase.

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