June 1990
Volume 31, Issue 6
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Articles  |   June 1990
HSV-2 alters retinal physiology and morphology bilaterally in mice.
Author Affiliations
  • D I Hamasaki
    William L. McKnight Vision Research Institute, Bascom Palmer Eye Institute, Miami, FL 33136.
  • S S Atherton
    William L. McKnight Vision Research Institute, Bascom Palmer Eye Institute, Miami, FL 33136.
  • R D Dix
    William L. McKnight Vision Research Institute, Bascom Palmer Eye Institute, Miami, FL 33136.
Investigative Ophthalmology & Visual Science June 1990, Vol.31, 1056-1069. doi:
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      D I Hamasaki, S S Atherton, R D Dix; HSV-2 alters retinal physiology and morphology bilaterally in mice.. Invest. Ophthalmol. Vis. Sci. 1990;31(6):1056-1069.

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Abstract

Anterior chamber inoculation of 10(4) PFU of the MS strain of HSV-2 resulted in physiologic and morphologic changes in the retina of the inoculated and the uninoculated eyes. In the inoculated eyes, electroretinogram (ERG) depression was first detected on day 3 and abolished ERGs on day 8 postinoculation (PI). The decrease in the ERGs was rapid and the time course was similar for all of the eyes. In spite of a 90% decrease in the amplitude of the b-wave, the retinal sensitivity did not change. Of 23 eyes tested on or after day 10 PI, none had normal, 4.3% had reduced, and 95.6% had abolished ERGs. In the uninoculated eyes, ERG depression was first detected on day 8 and abolished ERGs on day 12 PI. The course of the ERG depression was more variable, and some of the eyes showed a decrease in retinal sensitivity. Of the 22 eyes tested on or after day 17 PI, 18% had normal, 32% had reduced, and 50% had abolished ERGs. The majority (17/33) of the retinas of the inoculated eyes showed panretinal necrosis, although 7 of 33 retinas had pathology confined to the outer layers of the retina. In the uninoculated eyes, only 5 of 30 retinas were necrotic and 14 of 30 retinas had pathology limited to the outer layers of the retina. These observations suggested that the physiologic and morphologic changes progress through two stages: an early stage with reduced ERGs and pathology limited to the outer retinal layers, and a second stage in which the ERG is abolished and the pathologic changes extend into the inner retina. Not all retinas progress to the second stage.

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