September 1992
Volume 33, Issue 10
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Articles  |   September 1992
Oxiradical-dependent photoemission induced by a phacoemulsification probe.
Author Affiliations
  • S Shimmura
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
  • K Tsubota
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
  • Y Oguchi
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
  • D Fukumura
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
  • M Suematsu
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
  • M Tsuchiya
    Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
Investigative Ophthalmology & Visual Science September 1992, Vol.33, 2904-2907. doi:
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    • Get Citation

      S Shimmura, K Tsubota, Y Oguchi, D Fukumura, M Suematsu, M Tsuchiya; Oxiradical-dependent photoemission induced by a phacoemulsification probe.. Invest. Ophthalmol. Vis. Sci. 1992;33(10):2904-2907.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Oxygen free radical formation by conventional phacoemulsification devices has been postulated as a possible mechanism of corneal endothelial damage during surgery. To test this hypothesis, phacoemulsification probe-induced free radical production was visualized using a single photon-counting camera and an O(2-)-sensitive luciferin derivative, 2-methyl-6-[p-methoxyphenyl-3,7-dihydroimidazo [1,2-a]pyrazin-3-one (MCLA), which allows the visualization of spatial and temporal alterations in free radical production. Within 1 min after starting ultrasound emission, MCLA-dependent chemiluminescence was increased significantly, the intensity of which was maximal at the tip of the probe and tapered along a gradient toward distal portions. The chemiluminescence was suppressed significantly by adding either superoxide dismutase (300 U/ml) or sodium azide (20 mmol/l). By adding deuterium to the medium, MCLA-dependent chemiluminescence significantly increased, suggesting the involvement of singlet oxygen in the reaction.

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