September 1989
Volume 30, Issue 9
Free
Articles  |   September 1989
Quantitative analysis of wide-field specular microscopy. II. Precision of sampling from the central corneal endothelium.
Author Affiliations
  • L W Hirst
    Department of Surgery, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
  • K Yamauchi
    Department of Surgery, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
  • C Enger
    Department of Surgery, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
  • W Vogelpohl
    Department of Surgery, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
  • V Whittington
    Department of Surgery, University of Queensland, Princess Alexandra Hospital, Brisbane, Australia.
Investigative Ophthalmology & Visual Science September 1989, Vol.30, 1972-1979. doi:
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      L W Hirst, K Yamauchi, C Enger, W Vogelpohl, V Whittington; Quantitative analysis of wide-field specular microscopy. II. Precision of sampling from the central corneal endothelium.. Invest. Ophthalmol. Vis. Sci. 1989;30(9):1972-1979.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

The precision of the measurement of mean endothelial cell area obtained by sampling with small-field and wide-field specular microscopy from the central 4 mm of human corneal endothelium was studied by comparing endothelial cell parameters from individual specular micrographs in vivo to the results obtained by montaging the micrographs from the entire central 4 mm of the same corneas. The small samples were at least 10% from the true mean cell size of all cells of the central 4 mm in any endothelium other than that with the most homogeneous pattern. A new algorithm for sampling with these two specular microscopes will need to be derived to permit a more precise measure of the mean area of endothelial cells in the central 4 mm of the human corneal endothelium.

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