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L W Hirst, K Yamauchi, C Enger, W Vogelpohl, V Whittington; Quantitative analysis of wide-field specular microscopy. II. Precision of sampling from the central corneal endothelium.. Invest. Ophthalmol. Vis. Sci. 1989;30(9):1972-1979.
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The precision of the measurement of mean endothelial cell area obtained by sampling with small-field and wide-field specular microscopy from the central 4 mm of human corneal endothelium was studied by comparing endothelial cell parameters from individual specular micrographs in vivo to the results obtained by montaging the micrographs from the entire central 4 mm of the same corneas. The small samples were at least 10% from the true mean cell size of all cells of the central 4 mm in any endothelium other than that with the most homogeneous pattern. A new algorithm for sampling with these two specular microscopes will need to be derived to permit a more precise measure of the mean area of endothelial cells in the central 4 mm of the human corneal endothelium.
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