July 1990
Volume 31, Issue 7
Free
Articles  |   July 1990
In vitro susceptibility of newborn murine retinal cells to herpes simplex virus type 1 infection.
Author Affiliations
  • M J Merges
    Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland.
  • J A Whittum-Hudson
    Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Investigative Ophthalmology & Visual Science July 1990, Vol.31, 1224-1230. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      M J Merges, J A Whittum-Hudson; In vitro susceptibility of newborn murine retinal cells to herpes simplex virus type 1 infection.. Invest. Ophthalmol. Vis. Sci. 1990;31(7):1224-1230.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
This content is PDF only. Please click on the PDF icon to access.
Abstract

We have investigated the in vitro susceptibility of murine neural retinal cells to infection by herpes simplex virus Type 1 (HSV-1). Retinal cells obtained from newborn C57B1/6 mice were cultured for 6 days and infected with varying doses of HSV-1. Infection was determined by ABC immunoperoxidase staining of fixed cultures for HSV-1 antigens. Retinal neurons, including amacrine cells, were highly susceptible to infection, with 100% of the multipolar neurons expressing viral antigens after 12 hr of infection. Glial cells and retinal pigment epithelial (RPE) cells also were 100% infected within 12-16 hr. Photoreceptor infection was not as fast, but all surviving photoreceptor cells and their precursors became infected by 24-48 hr postinoculation. Since embryonic chick photoreceptors are highly resistant to HSV-1, these results demonstrate that mammalian (murine) photoreceptor cells differ from avian photoreceptor cells in their susceptibility to in vitro HSV-1 infection. In addition, our current results suggest that the in vivo resistance of adult C57B1/6 mice to herpetic retinitis may not reside at the level of the individual retinal cell populations, although apparent differences in susceptibility exist among the various retinal cell subpopulations.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×