September 1989
Volume 30, Issue 9
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Articles  |   September 1989
Near-ultraviolet circular dichroism of bovine high molecular weight alpha-crystallin.
Author Affiliations
  • J Liang
    Howe Laboratory of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston 02114.
  • M Rossi
    Howe Laboratory of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston 02114.
Investigative Ophthalmology & Visual Science September 1989, Vol.30, 2065-2068. doi:
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      J Liang, M Rossi; Near-ultraviolet circular dichroism of bovine high molecular weight alpha-crystallin.. Invest. Ophthalmol. Vis. Sci. 1989;30(9):2065-2068.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

High molecular weight (HMW) alpha-crystallin isolated from cow nucleus has a molecular weight greater than 5 x 10(6) Daltons. Its particle dimensions have been reported to be over 500 nm. It is derived mainly from low molecular weight (LMW) alpha-crystallin. The conformational change as LMW alpha-crystallin is converted into HMW alpha-crystallin was studied by near-ultraviolet circular dichroism (NUV-CD). The main concern in measuring CD for protein aggregates with particle dimensions as high as HMW alpha-crystallin and with an irregular shape is the contribution of differential scattering. The differential scattering becomes increasingly more important for particles with dimensions greater than 1/20th the wavelength of light. This contribution was minimized by increasing the acceptance half-angle of detection. The present data indicate that bovine HMW alpha-crystallin has a different tertiary structure from LMW alpha-crystallin.

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