January 1992
Volume 33, Issue 1
Free
Articles  |   January 1992
Retinal pigment epithelial cells secrete interleukin-6 in response to interleukin-1.
Author Affiliations
  • S R Planck
    Department of Medicine, Oregon Health Sciences University, Portland 97201-3098.
  • T T Dang
    Department of Medicine, Oregon Health Sciences University, Portland 97201-3098.
  • D Graves
    Department of Medicine, Oregon Health Sciences University, Portland 97201-3098.
  • D Tara
    Department of Medicine, Oregon Health Sciences University, Portland 97201-3098.
  • J C Ansel
    Department of Medicine, Oregon Health Sciences University, Portland 97201-3098.
  • J T Rosenbaum
    Department of Medicine, Oregon Health Sciences University, Portland 97201-3098.
Investigative Ophthalmology & Visual Science January 1992, Vol.33, 78-82. doi:
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    • Get Citation

      S R Planck, T T Dang, D Graves, D Tara, J C Ansel, J T Rosenbaum; Retinal pigment epithelial cells secrete interleukin-6 in response to interleukin-1.. Invest. Ophthalmol. Vis. Sci. 1992;33(1):78-82.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Interleukin-6 (IL-6) is a peptide whose properties include the ability to activate T-lymphocytes, stimulate the secretion of immunoglobulin, induce neuronal differentiation, and trigger the release of acute phase proteins. We have detected IL-6-like activity in conditioned medium from cultured human retinal pigment epithelial (RPE) cells with a bioassay based on the ability of IL-6 to induce the proliferation of murine B-9 plasmacytoma cells. Biologic activity increased approximately 90-fold when the cells were cultured in the presence of IL-1 alpha (30 units/ml). Western blot analysis confirmed that conditioned medium from IL-1 alpha-stimulated RPE cells contained peptides with molecular weights ranging between 19,000 and 30,000 and reactive with antibody to IL-6. Finally, Northern blot analysis indicated that cells cultured in the presence of interleukin-1 contained a 1.2 kilobase transcript that hybridized to a cDNA probe specific for IL-6 messenger RNA. IL-6 peptide on Western blots and mRNA on Northern blots were undetectable unless cells were cultured in the presence of IL-1 alpha. Although IL-6 is synthesized by a variety of cell types, this report is the first to detect its synthesis by an eye-specific cell type. Furthermore, these observations indicate that retinal pigment epithelial cells respond to IL-1, a cytokine that previously has been implicated in ocular inflammation.

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