May 1992
Volume 33, Issue 6
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Articles  |   May 1992
A quantitative assay of retrograde transported HSV in the trigeminal ganglion.
Author Affiliations
  • S R Carter
    Department of Anatomy and Neuroscience Program, University of California San Francisco 94143-0452.
  • L Pereira
    Department of Anatomy and Neuroscience Program, University of California San Francisco 94143-0452.
  • P Paz
    Department of Anatomy and Neuroscience Program, University of California San Francisco 94143-0452.
  • J H LaVail
    Department of Anatomy and Neuroscience Program, University of California San Francisco 94143-0452.
Investigative Ophthalmology & Visual Science May 1992, Vol.33, 1934-1939. doi:
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      S R Carter, L Pereira, P Paz, J H LaVail; A quantitative assay of retrograde transported HSV in the trigeminal ganglion.. Invest. Ophthalmol. Vis. Sci. 1992;33(6):1934-1939.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

The relationship between the dose of Herpes simplex virus type 1 (HSV) inoculated in the cornea and the amount of actively replicating virus recovered from mouse trigeminal ganglion cells 5 d after corneal scratch and inoculation was investigated with a tissue culture plaque assay. A dose response curve of productive viral replication was obtained. The estimated dose of HSV that produces half-maximal recovery of virus within the ganglion was 9.15 x 10(3) plaque forming units per eye, and the maximal amount of HSV recovered was 1.34 x 10(4) pfu per ganglion. This definition of infectivity as a function of dose will be useful for studying the effects of potential inhibitors of the binding, uptake, and transport of HSV by productively or latently infected trigeminal neurons.

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