March 1992
Volume 33, Issue 3
Free
Articles  |   March 1992
Endotoxin-induced uveitis in the rat. The significance of intraocular interleukin-6.
Author Affiliations
  • R Hoekzema
    Department of Ophthalmo-Immunology, Netherlands Ophthalmic Research Institute, Amsterdam.
  • C Verhagen
    Department of Ophthalmo-Immunology, Netherlands Ophthalmic Research Institute, Amsterdam.
  • M van Haren
    Department of Ophthalmo-Immunology, Netherlands Ophthalmic Research Institute, Amsterdam.
  • A Kijlstra
    Department of Ophthalmo-Immunology, Netherlands Ophthalmic Research Institute, Amsterdam.
Investigative Ophthalmology & Visual Science March 1992, Vol.33, 532-539. doi:
  • Views
  • PDF
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      R Hoekzema, C Verhagen, M van Haren, A Kijlstra; Endotoxin-induced uveitis in the rat. The significance of intraocular interleukin-6.. Invest. Ophthalmol. Vis. Sci. 1992;33(3):532-539.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

The potential role of interleukin-6 (IL-6) was studied as an inflammatory mediator of endotoxin (or lipopolysaccharide [LPS])-induced uveitis (EIU) in the rat. In young Lewis rats, levels of intraocular IL-6, but not serum IL-6, correlated with the severity of uveitis and with aqueous humor protein levels in response to foot pad injections of LPS (P less than 0.001). Adult Lewis rats did not develop uveitis and had no intraocular IL-6, although IL-6 was released systemically. Resistance to EIU and absence of IL-6 levels in the aqueous humor, despite the ability to release serum IL-6, also were observed in brown Norway rats, irrespective of age and weight. Intravitreal injection of as little as 1 ng of human recombinant IL-6 induced uveitis in young Lewis rats. In adult Lewis rats, and in young animals made tolerant to LPS, intravitreal IL-6 still caused substantial leakage of plasma proteins into the anterior chamber but no influx of inflammatory cells. As early as 2 hr after intravitreal injection of IL-6, immunohistochemical analysis showed invasion of the iris, corneal stroma, and anterior chamber by polymorphonuclear leukocytes (PMN) and expression of major histocompatibility complex (MHC) class II antigen in the retina by large cells that were macrophage-marker ED2 negative. This was followed by massive PMN infiltration of the retinal layers and vitreous. The MHC class II antigen expression of ciliary and iris epithelium occurred at a later stage (greater than 8 hr).(ABSTRACT TRUNCATED AT 250 WORDS)

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×