April 1992
Volume 33, Issue 5
Free
Articles  |   April 1992
Differential localization of dopamine D1 and D2 receptors in rat retina.
Author Affiliations
  • V T Tran
    Doheny Eye Institute, Los Angeles, CA 90033.
  • M Dickman
    Doheny Eye Institute, Los Angeles, CA 90033.
Investigative Ophthalmology & Visual Science April 1992, Vol.33, 1620-1626. doi:
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      V T Tran, M Dickman; Differential localization of dopamine D1 and D2 receptors in rat retina.. Invest. Ophthalmol. Vis. Sci. 1992;33(5):1620-1626.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Dopamine interacts with distinct receptors on different target neurons in the rat retina. Dopamine receptors were labeled in the rat retina by autoradiography using 3H-SCH-23390 and 3H-spiperone binding to retinal sections. The 3H-SCH-23390 binding to D1 receptors was most concentrated in the inner plexiform, inner nuclear, and ganglion cell layers; it was absent from the outer nuclear layer and the photoreceptor inner and outer segments. Competition studies indicated that 3H-SCH-23390 binding to the inner retina was inhibited with high affinities by the D1-specific agonist and antagonist, SKF-38393 and SCH-23390. The D2-specific compounds were ineffective in competing for 3H-SCH-23390 binding. The 3H-spiperone binding to D2 receptors, however, was most concentrated in the photoreceptor inner and outer segments and in the outer nuclear layer. The D2-specific agonist and antagonists, such as quinpirole, sulpiride, and eticlopride, competed for 3H-binding with high affinities; SCH-23390 and SKF-38393 were ineffective. The D2 receptors on the photoreceptors had a high affinity for clozapine but lower affinities for the modified benzamides. This is characteristic of a novel subtype of D2 receptors. Thus, D1 and D2 dopamine receptors are localized differentially in the rat retina to mediate different physiologic effects of dopamine.

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